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All rights reserved. http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/title BEL Framework Large Corpus Document http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/pav/authoredBy http://www.tkuhn.ch/bel2nanopub/RAHlWiOI5N3ADImhQ-WmF9bxltOgS6-HMUWq1o7888JpI#_6 http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/pav/version 20131211 http://www.tkuhn.ch/bel2nanopub/RAHlWiOI5N3ADImhQ-WmF9bxltOgS6-HMUWq1o7888JpI#_5 http://www.w3.org/ns/prov#value Previous studies have shown that human heat shock protein (hsp) 60 elicits a strong proinflammatory response in cells of the innate immune system with CD14, Toll-like receptor (TLR) 2, and TLR4 as mediators of signaling, but probably not of binding. In the present study, we directly demonstrate binding of hsp60 to the macrophage surface and find the binding receptor for hsp60 different from the previously described common receptor for several other heat shock proteins, including hsp70, hsp90, and gp96. Fluorescence-labeled human hsp60 bound to cell surfaces of the murine macrophage lines J774 A.1 and RAW264.7 and to mouse bone marrow-derived macrophages. By flow cytometry, we could demonstrate for the first time that hsp60 binding to macrophages occurred at submicromolar concentrations, is saturable, and can be competed by unlabeled hsp60, but not by unrelated proteins, thus confirming the classic characteristics of specific ligand-receptor interactions. Binding of hsp60 at 4 degrees C was followed by endocytosis at 37 degrees C. Hsp60 binding to macrophages could not be competed by excess hsp70, hsp90, or gp96, all of which share the alpha(2)-macroglobulin receptor as binding site. Hsp60 binding occurred in the absence of surface TLR4. However, no cytokine response was induced by hsp60 in TLR4-deficient macrophages. We conclude that hsp60 binds to a stereo-specific receptor on macrophages, and that different surface molecules are engaged in binding and signal transduction. Furthermore, the binding site for hsp60 is separate from the common receptor for hsp70, hsp90, and gp96, which suggests an independent role of hsp60 as danger Ag and in immunoregulation. http://www.tkuhn.ch/bel2nanopub/RAHlWiOI5N3ADImhQ-WmF9bxltOgS6-HMUWq1o7888JpI#_5 http://www.w3.org/ns/prov#wasQuotedFrom http://www.ncbi.nlm.nih.gov/pubmed/11777948 http://www.tkuhn.ch/bel2nanopub/RAHlWiOI5N3ADImhQ-WmF9bxltOgS6-HMUWq1o7888JpI#_6 http://www.w3.org/2000/01/rdf-schema#label Selventa http://www.tkuhn.ch/bel2nanopub/RAHlWiOI5N3ADImhQ-WmF9bxltOgS6-HMUWq1o7888JpI#assertion http://www.w3.org/ns/prov#hadPrimarySource http://www.ncbi.nlm.nih.gov/pubmed/11777948 http://www.tkuhn.ch/bel2nanopub/RAHlWiOI5N3ADImhQ-WmF9bxltOgS6-HMUWq1o7888JpI#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://www.tkuhn.ch/bel2nanopub/RAHlWiOI5N3ADImhQ-WmF9bxltOgS6-HMUWq1o7888JpI#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://www.tkuhn.ch/bel2nanopub/RAHlWiOI5N3ADImhQ-WmF9bxltOgS6-HMUWq1o7888JpI#_5 http://www.tkuhn.ch/bel2nanopub/RAHlWiOI5N3ADImhQ-WmF9bxltOgS6-HMUWq1o7888JpI#pubinfo http://www.tkuhn.ch/bel2nanopub/RAHlWiOI5N3ADImhQ-WmF9bxltOgS6-HMUWq1o7888JpI http://purl.org/dc/terms/created 2014-07-03T14:31:45.711+02:00 http://www.tkuhn.ch/bel2nanopub/RAHlWiOI5N3ADImhQ-WmF9bxltOgS6-HMUWq1o7888JpI http://purl.org/pav/createdBy http://orcid.org/0000-0001-6818-334X http://www.tkuhn.ch/bel2nanopub/RAHlWiOI5N3ADImhQ-WmF9bxltOgS6-HMUWq1o7888JpI http://purl.org/pav/createdBy http://orcid.org/0000-0002-1267-0234