http://www.tkuhn.ch/bel2nanopub/RAHKshNp7EpqKEOaXAnKDe2KfBDVxBEYA076VGRBTpdrU#Head http://www.tkuhn.ch/bel2nanopub/RAHKshNp7EpqKEOaXAnKDe2KfBDVxBEYA076VGRBTpdrU http://www.nanopub.org/nschema#hasAssertion http://www.tkuhn.ch/bel2nanopub/RAHKshNp7EpqKEOaXAnKDe2KfBDVxBEYA076VGRBTpdrU#assertion http://www.tkuhn.ch/bel2nanopub/RAHKshNp7EpqKEOaXAnKDe2KfBDVxBEYA076VGRBTpdrU http://www.nanopub.org/nschema#hasProvenance http://www.tkuhn.ch/bel2nanopub/RAHKshNp7EpqKEOaXAnKDe2KfBDVxBEYA076VGRBTpdrU#provenance http://www.tkuhn.ch/bel2nanopub/RAHKshNp7EpqKEOaXAnKDe2KfBDVxBEYA076VGRBTpdrU http://www.nanopub.org/nschema#hasPublicationInfo http://www.tkuhn.ch/bel2nanopub/RAHKshNp7EpqKEOaXAnKDe2KfBDVxBEYA076VGRBTpdrU#pubinfo http://www.tkuhn.ch/bel2nanopub/RAHKshNp7EpqKEOaXAnKDe2KfBDVxBEYA076VGRBTpdrU http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.nanopub.org/nschema#Nanopublication http://www.tkuhn.ch/bel2nanopub/RAHKshNp7EpqKEOaXAnKDe2KfBDVxBEYA076VGRBTpdrU#assertion http://www.tkuhn.ch/bel2nanopub/RAHKshNp7EpqKEOaXAnKDe2KfBDVxBEYA076VGRBTpdrU#_1 http://purl.obolibrary.org/obo/RO_0002204 http://www.informatics.jax.org/marker/MGI:104887 http://www.tkuhn.ch/bel2nanopub/RAHKshNp7EpqKEOaXAnKDe2KfBDVxBEYA076VGRBTpdrU#_1 http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.ebi.ac.uk/chebi/searchId.do?chebiId=CHEBI_36080 http://www.tkuhn.ch/bel2nanopub/RAHKshNp7EpqKEOaXAnKDe2KfBDVxBEYA076VGRBTpdrU#_2 http://purl.obolibrary.org/obo/BFO_0000066 http://purl.bioontology.org/ontology/MSH/D042783 http://www.tkuhn.ch/bel2nanopub/RAHKshNp7EpqKEOaXAnKDe2KfBDVxBEYA076VGRBTpdrU#_2 http://purl.obolibrary.org/obo/BFO_0000066 http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=10090 http://www.tkuhn.ch/bel2nanopub/RAHKshNp7EpqKEOaXAnKDe2KfBDVxBEYA076VGRBTpdrU#_2 http://www.w3.org/1999/02/22-rdf-syntax-ns#object http://www.ebi.ac.uk/chebi/searchId.do?chebiId=26523 http://www.tkuhn.ch/bel2nanopub/RAHKshNp7EpqKEOaXAnKDe2KfBDVxBEYA076VGRBTpdrU#_2 http://www.w3.org/1999/02/22-rdf-syntax-ns#predicate http://www.selventa.com/vocabulary/decreases http://www.tkuhn.ch/bel2nanopub/RAHKshNp7EpqKEOaXAnKDe2KfBDVxBEYA076VGRBTpdrU#_2 http://www.w3.org/1999/02/22-rdf-syntax-ns#subject http://www.tkuhn.ch/bel2nanopub/RAHKshNp7EpqKEOaXAnKDe2KfBDVxBEYA076VGRBTpdrU#_1 http://www.tkuhn.ch/bel2nanopub/RAHKshNp7EpqKEOaXAnKDe2KfBDVxBEYA076VGRBTpdrU#_2 http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.w3.org/1999/02/22-rdf-syntax-ns#Statement http://www.tkuhn.ch/bel2nanopub/RAHKshNp7EpqKEOaXAnKDe2KfBDVxBEYA076VGRBTpdrU#assertion http://www.w3.org/2000/01/rdf-schema#label p(MGI:Gpx1) -| a(CHEBI:"reactive oxygen species") http://www.tkuhn.ch/bel2nanopub/RAHKshNp7EpqKEOaXAnKDe2KfBDVxBEYA076VGRBTpdrU#provenance http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/description Approximately 61,000 statements. http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/rights Copyright (c) 2011-2012, Selventa. All rights reserved. http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/title BEL Framework Large Corpus Document http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/pav/authoredBy http://www.tkuhn.ch/bel2nanopub/RAHKshNp7EpqKEOaXAnKDe2KfBDVxBEYA076VGRBTpdrU#_4 http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/pav/version 1.4 http://www.tkuhn.ch/bel2nanopub/RAHKshNp7EpqKEOaXAnKDe2KfBDVxBEYA076VGRBTpdrU#_3 http://www.w3.org/ns/prov#value OBJECTIVE: We tested the hypothesis that deficiency of cellular glutathione peroxidase (GPx-1) enhances susceptibility to endothelial dysfunction in mice with moderate hyperhomocysteinemia. METHODS AND RESULTS: Mice that were wild type (Gpx1+/+), heterozygous (Gpx1+/-), or homozygous (Gpx1-/-) for the mutated Gpx1 allele were fed a control diet or a high-methionine diet for 17 weeks. Plasma total homocysteine was elevated in mice on the high-methionine diet compared with mice on the control diet (23+/-3 versus 6+/-0.3 micromol/L, respectively; P<0.001) and was not influenced by Gpx1 genotype. In mice fed the control diet, maximal relaxation of the aorta in response to the endothelium-dependent dilator acetylcholine (10(-5) mol/L) was similar in Gpx1+/+, Gpx1+/-, and Gpx1-/- mice, but relaxation to lower concentrations of acetylcholine was selectively impaired in Gpx1-/- mice (P<0.05 versus Gpx1+/+ mice). In mice fed the high-methionine diet, relaxation to low and high concentrations of acetylcholine was impaired in Gpx1-/- mice (maximal relaxation 73+/-6% in Gpx1-/- mice versus 90+/-2% in Gpx1+/+ mice, P<0.05). No differences in vasorelaxation to nitroprusside or papaverine were observed between Gpx1+/+ and Gpx1-/- mice fed either diet. Dihydroethidium fluorescence, a marker of superoxide, was elevated in Gpx1-/- mice fed the high-methionine diet (P<0.05 versus Gpx1+/+ mice fed the control diet). CONCLUSIONS: These findings demonstrate that deficiency of GPx-1 exacerbates endothelial dysfunction in hyperhomocysteinemic mice and provides support for the hypothesis that hyperhomocysteinemia contributes to endothelial dysfunction through a peroxide-dependent oxidative mechanism. http://www.tkuhn.ch/bel2nanopub/RAHKshNp7EpqKEOaXAnKDe2KfBDVxBEYA076VGRBTpdrU#_3 http://www.w3.org/ns/prov#wasQuotedFrom http://www.ncbi.nlm.nih.gov/pubmed/12482825 http://www.tkuhn.ch/bel2nanopub/RAHKshNp7EpqKEOaXAnKDe2KfBDVxBEYA076VGRBTpdrU#_4 http://www.w3.org/2000/01/rdf-schema#label Selventa http://www.tkuhn.ch/bel2nanopub/RAHKshNp7EpqKEOaXAnKDe2KfBDVxBEYA076VGRBTpdrU#assertion http://www.w3.org/ns/prov#hadPrimarySource http://www.ncbi.nlm.nih.gov/pubmed/12482825 http://www.tkuhn.ch/bel2nanopub/RAHKshNp7EpqKEOaXAnKDe2KfBDVxBEYA076VGRBTpdrU#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://www.tkuhn.ch/bel2nanopub/RAHKshNp7EpqKEOaXAnKDe2KfBDVxBEYA076VGRBTpdrU#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://www.tkuhn.ch/bel2nanopub/RAHKshNp7EpqKEOaXAnKDe2KfBDVxBEYA076VGRBTpdrU#_3 http://www.tkuhn.ch/bel2nanopub/RAHKshNp7EpqKEOaXAnKDe2KfBDVxBEYA076VGRBTpdrU#pubinfo http://www.tkuhn.ch/bel2nanopub/RAHKshNp7EpqKEOaXAnKDe2KfBDVxBEYA076VGRBTpdrU http://purl.org/dc/terms/created 2014-07-03T14:30:07.920+02:00 http://www.tkuhn.ch/bel2nanopub/RAHKshNp7EpqKEOaXAnKDe2KfBDVxBEYA076VGRBTpdrU http://purl.org/pav/createdBy http://orcid.org/0000-0001-6818-334X http://www.tkuhn.ch/bel2nanopub/RAHKshNp7EpqKEOaXAnKDe2KfBDVxBEYA076VGRBTpdrU http://purl.org/pav/createdBy http://orcid.org/0000-0002-1267-0234