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All rights reserved. http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/title BEL Framework Large Corpus Document http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/pav/authoredBy http://www.tkuhn.ch/bel2nanopub/RAFXUweOyof4d0nYpoIwjPZTidRFSxL9kuOmcM1eLdY10#_6 http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/pav/version 1.4 http://www.tkuhn.ch/bel2nanopub/RAFXUweOyof4d0nYpoIwjPZTidRFSxL9kuOmcM1eLdY10#_5 http://www.w3.org/ns/prov#value In the studies presented here, we identify the presence of a second functional Pitx1 region spanning positions -73 to -52 in the rat LHbeta gene promoter. Based on electrophoretic mobility shift assay, Pitx1 binds to both the initially described 5'Pitx1 site as well as this putative 3'Pitx1 region. In transient transfection analysis, mutation of the LHbeta-3'Pitx1 site significantly blunted Pitx1 responsiveness, with elimination of the Pitx1 response in a construct containing mutations in both Pitx1 cis-elements. http://www.tkuhn.ch/bel2nanopub/RAFXUweOyof4d0nYpoIwjPZTidRFSxL9kuOmcM1eLdY10#_5 http://www.w3.org/ns/prov#wasQuotedFrom http://www.ncbi.nlm.nih.gov/pubmed/16087728 http://www.tkuhn.ch/bel2nanopub/RAFXUweOyof4d0nYpoIwjPZTidRFSxL9kuOmcM1eLdY10#_6 http://www.w3.org/2000/01/rdf-schema#label Selventa http://www.tkuhn.ch/bel2nanopub/RAFXUweOyof4d0nYpoIwjPZTidRFSxL9kuOmcM1eLdY10#assertion http://www.w3.org/ns/prov#hadPrimarySource http://www.ncbi.nlm.nih.gov/pubmed/16087728 http://www.tkuhn.ch/bel2nanopub/RAFXUweOyof4d0nYpoIwjPZTidRFSxL9kuOmcM1eLdY10#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://www.tkuhn.ch/bel2nanopub/RAFXUweOyof4d0nYpoIwjPZTidRFSxL9kuOmcM1eLdY10#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://www.tkuhn.ch/bel2nanopub/RAFXUweOyof4d0nYpoIwjPZTidRFSxL9kuOmcM1eLdY10#_5 http://www.tkuhn.ch/bel2nanopub/RAFXUweOyof4d0nYpoIwjPZTidRFSxL9kuOmcM1eLdY10#pubinfo http://www.tkuhn.ch/bel2nanopub/RAFXUweOyof4d0nYpoIwjPZTidRFSxL9kuOmcM1eLdY10 http://purl.org/dc/terms/created 2014-07-03T14:30:37.314+02:00 http://www.tkuhn.ch/bel2nanopub/RAFXUweOyof4d0nYpoIwjPZTidRFSxL9kuOmcM1eLdY10 http://purl.org/pav/createdBy http://orcid.org/0000-0001-6818-334X http://www.tkuhn.ch/bel2nanopub/RAFXUweOyof4d0nYpoIwjPZTidRFSxL9kuOmcM1eLdY10 http://purl.org/pav/createdBy http://orcid.org/0000-0002-1267-0234