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All rights reserved. http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/title BEL Framework Large Corpus Document http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/pav/authoredBy http://www.tkuhn.ch/bel2nanopub/RAE_INR0BRjaonDdka52tVRPs8PQ3z_16Sn5z0JUfxbk0#_6 http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/pav/version 1.4 http://www.tkuhn.ch/bel2nanopub/RAE_INR0BRjaonDdka52tVRPs8PQ3z_16Sn5z0JUfxbk0#_5 http://www.w3.org/ns/prov#value co-localization of hA1AT and active caspase-3 by immunofluorescence microscopy (Figure 3a) suggested an interaction between the hA1AT with the proapoptotic cysteine protease caspase-3 in mouse and rat microvascular pulmonary endothelial cells treated with staurosporine.....was further tested biochemically as mouse lung endothelial cells pretreated with hA1AT and exposed to staurosporine were evaluated by immunoprecipitation with active caspase-3 antibodies followed by immunoblotting with hA1AT-specific antibodies. This study confirmed an interaction between hA1AT and caspase-3 (Figure 3b) in lung primary endothelial cells....A complex containing hA1AT and caspase-3 was detected by incubating hA1AT with His-tagged caspase-3 for 1 hour, followed by electrophoresis and immunoblotting sequentially for A1AT and then His-tag (Figure 4a). http://www.tkuhn.ch/bel2nanopub/RAE_INR0BRjaonDdka52tVRPs8PQ3z_16Sn5z0JUfxbk0#_5 http://www.w3.org/ns/prov#wasQuotedFrom http://www.ncbi.nlm.nih.gov/pubmed/17003475 http://www.tkuhn.ch/bel2nanopub/RAE_INR0BRjaonDdka52tVRPs8PQ3z_16Sn5z0JUfxbk0#_6 http://www.w3.org/2000/01/rdf-schema#label Selventa http://www.tkuhn.ch/bel2nanopub/RAE_INR0BRjaonDdka52tVRPs8PQ3z_16Sn5z0JUfxbk0#assertion http://www.w3.org/ns/prov#hadPrimarySource http://www.ncbi.nlm.nih.gov/pubmed/17003475 http://www.tkuhn.ch/bel2nanopub/RAE_INR0BRjaonDdka52tVRPs8PQ3z_16Sn5z0JUfxbk0#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://www.tkuhn.ch/bel2nanopub/RAE_INR0BRjaonDdka52tVRPs8PQ3z_16Sn5z0JUfxbk0#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://www.tkuhn.ch/bel2nanopub/RAE_INR0BRjaonDdka52tVRPs8PQ3z_16Sn5z0JUfxbk0#_5 http://www.tkuhn.ch/bel2nanopub/RAE_INR0BRjaonDdka52tVRPs8PQ3z_16Sn5z0JUfxbk0#pubinfo http://www.tkuhn.ch/bel2nanopub/RAE_INR0BRjaonDdka52tVRPs8PQ3z_16Sn5z0JUfxbk0 http://purl.org/dc/terms/created 2014-07-03T14:30:46.226+02:00 http://www.tkuhn.ch/bel2nanopub/RAE_INR0BRjaonDdka52tVRPs8PQ3z_16Sn5z0JUfxbk0 http://purl.org/pav/createdBy http://orcid.org/0000-0001-6818-334X http://www.tkuhn.ch/bel2nanopub/RAE_INR0BRjaonDdka52tVRPs8PQ3z_16Sn5z0JUfxbk0 http://purl.org/pav/createdBy http://orcid.org/0000-0002-1267-0234