@prefix obo: <http://purl.obolibrary.org/obo/> .
@prefix dce: <http://purl.org/dc/elements/1.1/> .
@prefix pplan: <http://purl.org/net/p-plan#> .
@prefix this: <http://purl.org/np/RAEJO3rzfeXAzRdHDEfPk7hsm-7SF02Ud4J02S9F1lbng> .
@prefix sub: <http://purl.org/np/RAEJO3rzfeXAzRdHDEfPk7hsm-7SF02Ud4J02S9F1lbng#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix prov: <http://www.w3.org/ns/prov#> .

sub:Head {
  this: np:hasAssertion sub:assertion;
    np:hasProvenance sub:provenance;
    np:hasPublicationInfo sub:pubInfo;
    a np:Nanopublication .
}

sub:assertion {
  <http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C62218> rdfs:label "70% ethanol" .
  
  obo:CHEBI_16236 rdfs:label "70% ethanol" .
  
  obo:UBERON_0036018 rdfs:label "pellet" .
  
  sub:step dce:description "Wash the pellet with 70% ethanol";
    a pplan:Step;
    bs:bioSampleUsed obo:UBERON_0036018;
    bs:reagentUsed <http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C62218>, obo:CHEBI_16236 .
}

sub:provenance {
  sub:assertion pav:createdWith <https://nanotate.bitsfetch.com>;
    prov:generatedAtTime "2021-03-10T10:37:44.052876"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/alejandra.sandoval> .
}

sub:pubInfo {
  sub:assertion prov:wasDerivedFrom authority:JFHRYnJREeu3RzuSos6ktg, authority:V0bmZnJREeug3jf_bSWzNg,
      authority:dZlmXHJREeuNGeMzeoF4Bg .
  
  sub:sig npx:hasAlgorithm "RSA";
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB";
    npx:hasSignature "GZI173ZtAVKGiTeRvj9nobQb6X0tE55erwH89dVBhd+N6EoqofiLXJQgWiOP5nCcIxwoiocqI6RCqhafe/AWSXfgdaf+jjvwCw4K0O5kv21jJjJ/iig8Olb0876abNrfiokFe13/DGLFxiDeIOleP4AWJ1KR2Urc0+QTbp3DxrE=";
    npx:hasSignatureTarget this: .
  
  this: npx:introduces sub:step;
    prov:generatedAtTime "2021-03-10T10:37:44.052876"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/alejandra.sandoval> .
}