http://www.tkuhn.ch/bel2nanopub/RADypG8ORilvLx_JZk1lfXVtviqijtGcFOK7AulQcMjLY#Head http://www.tkuhn.ch/bel2nanopub/RADypG8ORilvLx_JZk1lfXVtviqijtGcFOK7AulQcMjLY http://www.nanopub.org/nschema#hasAssertion http://www.tkuhn.ch/bel2nanopub/RADypG8ORilvLx_JZk1lfXVtviqijtGcFOK7AulQcMjLY#assertion http://www.tkuhn.ch/bel2nanopub/RADypG8ORilvLx_JZk1lfXVtviqijtGcFOK7AulQcMjLY http://www.nanopub.org/nschema#hasProvenance http://www.tkuhn.ch/bel2nanopub/RADypG8ORilvLx_JZk1lfXVtviqijtGcFOK7AulQcMjLY#provenance http://www.tkuhn.ch/bel2nanopub/RADypG8ORilvLx_JZk1lfXVtviqijtGcFOK7AulQcMjLY http://www.nanopub.org/nschema#hasPublicationInfo http://www.tkuhn.ch/bel2nanopub/RADypG8ORilvLx_JZk1lfXVtviqijtGcFOK7AulQcMjLY#pubinfo http://www.tkuhn.ch/bel2nanopub/RADypG8ORilvLx_JZk1lfXVtviqijtGcFOK7AulQcMjLY http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.nanopub.org/nschema#Nanopublication http://www.tkuhn.ch/bel2nanopub/RADypG8ORilvLx_JZk1lfXVtviqijtGcFOK7AulQcMjLY#assertion http://www.tkuhn.ch/bel2nanopub/RADypG8ORilvLx_JZk1lfXVtviqijtGcFOK7AulQcMjLY#_1 http://purl.obolibrary.org/obo/RO_0002204 http://www.genenames.org/cgi-bin/gene_symbol_report?hgnc_id=735 http://www.tkuhn.ch/bel2nanopub/RADypG8ORilvLx_JZk1lfXVtviqijtGcFOK7AulQcMjLY#_1 http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.ebi.ac.uk/chebi/searchId.do?chebiId=CHEBI_33697 http://www.tkuhn.ch/bel2nanopub/RADypG8ORilvLx_JZk1lfXVtviqijtGcFOK7AulQcMjLY#_2 http://purl.obolibrary.org/obo/BFO_0000066 http://purl.obolibrary.org/obo/EFO_0002795 http://www.tkuhn.ch/bel2nanopub/RADypG8ORilvLx_JZk1lfXVtviqijtGcFOK7AulQcMjLY#_2 http://purl.obolibrary.org/obo/BFO_0000066 http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=9606 http://www.tkuhn.ch/bel2nanopub/RADypG8ORilvLx_JZk1lfXVtviqijtGcFOK7AulQcMjLY#_2 http://www.w3.org/1999/02/22-rdf-syntax-ns#object http://www.tkuhn.ch/bel2nanopub/RADypG8ORilvLx_JZk1lfXVtviqijtGcFOK7AulQcMjLY#_1 http://www.tkuhn.ch/bel2nanopub/RADypG8ORilvLx_JZk1lfXVtviqijtGcFOK7AulQcMjLY#_2 http://www.w3.org/1999/02/22-rdf-syntax-ns#predicate http://www.selventa.com/vocabulary/decreases http://www.tkuhn.ch/bel2nanopub/RADypG8ORilvLx_JZk1lfXVtviqijtGcFOK7AulQcMjLY#_2 http://www.w3.org/1999/02/22-rdf-syntax-ns#subject http://www.ebi.ac.uk/chebi/searchId.do?chebiId=7583 http://www.tkuhn.ch/bel2nanopub/RADypG8ORilvLx_JZk1lfXVtviqijtGcFOK7AulQcMjLY#_2 http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.w3.org/1999/02/22-rdf-syntax-ns#Statement http://www.tkuhn.ch/bel2nanopub/RADypG8ORilvLx_JZk1lfXVtviqijtGcFOK7AulQcMjLY#assertion http://www.w3.org/2000/01/rdf-schema#label a(CHEBI:"nitric oxide") -| r(HGNC:ASAH1) http://www.tkuhn.ch/bel2nanopub/RADypG8ORilvLx_JZk1lfXVtviqijtGcFOK7AulQcMjLY#provenance http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/description Approximately 61,000 statements. http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/rights Copyright (c) 2011-2012, Selventa. All rights reserved. http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/title BEL Framework Large Corpus Document http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/pav/authoredBy http://www.tkuhn.ch/bel2nanopub/RADypG8ORilvLx_JZk1lfXVtviqijtGcFOK7AulQcMjLY#_4 http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/pav/version 20131211 http://www.tkuhn.ch/bel2nanopub/RADypG8ORilvLx_JZk1lfXVtviqijtGcFOK7AulQcMjLY#_3 http://www.w3.org/ns/prov#value The present study tested the hypothesis that acute increases in nitric oxide (NO) exert substantial influences on gene transcription in endothelial cells (ECs) via guanylyl cyclase (GC). Human umbilical veins ECs (HUVECs) were exposed to 0.1, 1, and 10 mM of sodium nitroprusside (SNP) for 4 h and to 1 mM SNP or 250 microM of (Z)-1[N-(2-aminoethyl)-N-(2-ammonioethyl)amino]diazen-1-ium-1,2-diolate (DETA-NONOate) for 2, 4, 8, and 24 h. Also, cells were exposed to DETA-NONOate in the presence and absence of the GC inhibitor 1H-[1,2,4]oxadiazolo-[4,3-a]quinoxalin-1-one (ODQ; 10 microM) for 4 h. RNA was isolated, reverse transcribed, Cy3 and Cy5 labeled, and analyzed using cDNA microarrays. Increasing doses of SNP predominantly depressed gene expression in HUVECs. Gene function was related to growth, adhesion, and cell structure. DETA-NONOate evoked a wave of expression changes (maximum at 4 h), with a remarkable downregulation of the transcription factors MSX1, RELB, and Egr-1. Both SNP- and DETA-NONOate-induced gene expression had faded after 24 h, despite continued elevation of cGMP in the medium. Coadministration of ODQ decreased many, but not all, of the transcriptional responses to DETA-NONOate. NO pronouncedly depressed EC gene expression, in particular of transcription factors. The observation that many, but not all, transcriptional changes induced by NO could be inhibited by inhibition of GC indicates the presence of GC-independent NO actions on gene expression. Thus EC gene expression responds to NO; however, the transcriptional response fades during prolonged exposure. This could allow the EC to respond to increased shear, without vigorous changes in gene expression. http://www.tkuhn.ch/bel2nanopub/RADypG8ORilvLx_JZk1lfXVtviqijtGcFOK7AulQcMjLY#_3 http://www.w3.org/ns/prov#wasQuotedFrom http://www.ncbi.nlm.nih.gov/pubmed/15242832 http://www.tkuhn.ch/bel2nanopub/RADypG8ORilvLx_JZk1lfXVtviqijtGcFOK7AulQcMjLY#_4 http://www.w3.org/2000/01/rdf-schema#label Selventa http://www.tkuhn.ch/bel2nanopub/RADypG8ORilvLx_JZk1lfXVtviqijtGcFOK7AulQcMjLY#assertion http://www.w3.org/ns/prov#hadPrimarySource http://www.ncbi.nlm.nih.gov/pubmed/15242832 http://www.tkuhn.ch/bel2nanopub/RADypG8ORilvLx_JZk1lfXVtviqijtGcFOK7AulQcMjLY#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://www.tkuhn.ch/bel2nanopub/RADypG8ORilvLx_JZk1lfXVtviqijtGcFOK7AulQcMjLY#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://www.tkuhn.ch/bel2nanopub/RADypG8ORilvLx_JZk1lfXVtviqijtGcFOK7AulQcMjLY#_3 http://www.tkuhn.ch/bel2nanopub/RADypG8ORilvLx_JZk1lfXVtviqijtGcFOK7AulQcMjLY#pubinfo http://www.tkuhn.ch/bel2nanopub/RADypG8ORilvLx_JZk1lfXVtviqijtGcFOK7AulQcMjLY http://purl.org/dc/terms/created 2014-07-03T14:32:25.366+02:00 http://www.tkuhn.ch/bel2nanopub/RADypG8ORilvLx_JZk1lfXVtviqijtGcFOK7AulQcMjLY http://purl.org/pav/createdBy http://orcid.org/0000-0001-6818-334X http://www.tkuhn.ch/bel2nanopub/RADypG8ORilvLx_JZk1lfXVtviqijtGcFOK7AulQcMjLY http://purl.org/pav/createdBy http://orcid.org/0000-0002-1267-0234