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All rights reserved. http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/title BEL Framework Large Corpus Document http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/pav/authoredBy http://www.tkuhn.ch/bel2nanopub/RADsVF-6IGBtXxKTC5ZSLmrywM5NxisiYSged1vcyGGoA#_4 http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/pav/version 20131211 http://www.tkuhn.ch/bel2nanopub/RADsVF-6IGBtXxKTC5ZSLmrywM5NxisiYSged1vcyGGoA#_3 http://www.w3.org/ns/prov#value In rats, the CYP4F family consists of four related genes, CYP4F1, CYP4F4, CYP4F5, and CYP4F6. Here we report development of fluorescent real-time quantitative polymerase chain reaction assays (TaqMan), which allow us to carry out specific quantitation of mRNA expression of all four members of this subfamily. Since no inducers for the CYP4F family are known to date, we validated these assays using clofibrate, a known suppressor of rat CYP4Fs. Additionally, Northern blot hybridization was carried out to validate these assays. http://www.tkuhn.ch/bel2nanopub/RADsVF-6IGBtXxKTC5ZSLmrywM5NxisiYSged1vcyGGoA#_3 http://www.w3.org/ns/prov#wasQuotedFrom http://www.ncbi.nlm.nih.gov/pubmed/12167568 http://www.tkuhn.ch/bel2nanopub/RADsVF-6IGBtXxKTC5ZSLmrywM5NxisiYSged1vcyGGoA#_4 http://www.w3.org/2000/01/rdf-schema#label Selventa http://www.tkuhn.ch/bel2nanopub/RADsVF-6IGBtXxKTC5ZSLmrywM5NxisiYSged1vcyGGoA#assertion http://www.w3.org/ns/prov#hadPrimarySource http://www.ncbi.nlm.nih.gov/pubmed/12167568 http://www.tkuhn.ch/bel2nanopub/RADsVF-6IGBtXxKTC5ZSLmrywM5NxisiYSged1vcyGGoA#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://www.tkuhn.ch/bel2nanopub/RADsVF-6IGBtXxKTC5ZSLmrywM5NxisiYSged1vcyGGoA#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://www.tkuhn.ch/bel2nanopub/RADsVF-6IGBtXxKTC5ZSLmrywM5NxisiYSged1vcyGGoA#_3 http://www.tkuhn.ch/bel2nanopub/RADsVF-6IGBtXxKTC5ZSLmrywM5NxisiYSged1vcyGGoA#pubinfo http://www.tkuhn.ch/bel2nanopub/RADsVF-6IGBtXxKTC5ZSLmrywM5NxisiYSged1vcyGGoA http://purl.org/dc/terms/created 2014-07-03T14:31:52.311+02:00 http://www.tkuhn.ch/bel2nanopub/RADsVF-6IGBtXxKTC5ZSLmrywM5NxisiYSged1vcyGGoA http://purl.org/pav/createdBy http://orcid.org/0000-0001-6818-334X http://www.tkuhn.ch/bel2nanopub/RADsVF-6IGBtXxKTC5ZSLmrywM5NxisiYSged1vcyGGoA http://purl.org/pav/createdBy http://orcid.org/0000-0002-1267-0234