http://www.tkuhn.ch/bel2nanopub/RADQYp1-yuP0Xt2qY1J_TCwufxjKzgtRM3a9ac7FjZphU#Head http://www.tkuhn.ch/bel2nanopub/RADQYp1-yuP0Xt2qY1J_TCwufxjKzgtRM3a9ac7FjZphU http://www.nanopub.org/nschema#hasAssertion http://www.tkuhn.ch/bel2nanopub/RADQYp1-yuP0Xt2qY1J_TCwufxjKzgtRM3a9ac7FjZphU#assertion http://www.tkuhn.ch/bel2nanopub/RADQYp1-yuP0Xt2qY1J_TCwufxjKzgtRM3a9ac7FjZphU http://www.nanopub.org/nschema#hasProvenance http://www.tkuhn.ch/bel2nanopub/RADQYp1-yuP0Xt2qY1J_TCwufxjKzgtRM3a9ac7FjZphU#provenance http://www.tkuhn.ch/bel2nanopub/RADQYp1-yuP0Xt2qY1J_TCwufxjKzgtRM3a9ac7FjZphU http://www.nanopub.org/nschema#hasPublicationInfo http://www.tkuhn.ch/bel2nanopub/RADQYp1-yuP0Xt2qY1J_TCwufxjKzgtRM3a9ac7FjZphU#pubinfo http://www.tkuhn.ch/bel2nanopub/RADQYp1-yuP0Xt2qY1J_TCwufxjKzgtRM3a9ac7FjZphU http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.nanopub.org/nschema#Nanopublication http://www.tkuhn.ch/bel2nanopub/RADQYp1-yuP0Xt2qY1J_TCwufxjKzgtRM3a9ac7FjZphU#assertion http://www.tkuhn.ch/bel2nanopub/RADQYp1-yuP0Xt2qY1J_TCwufxjKzgtRM3a9ac7FjZphU#_1 http://purl.obolibrary.org/obo/RO_0002204 http://www.informatics.jax.org/marker/MGI:5000466 http://www.tkuhn.ch/bel2nanopub/RADQYp1-yuP0Xt2qY1J_TCwufxjKzgtRM3a9ac7FjZphU#_1 http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.ebi.ac.uk/chebi/searchId.do?chebiId=CHEBI_33697 http://www.tkuhn.ch/bel2nanopub/RADQYp1-yuP0Xt2qY1J_TCwufxjKzgtRM3a9ac7FjZphU#_2 http://purl.obolibrary.org/obo/BFO_0000066 http://purl.obolibrary.org/obo/UBERON_0001276 http://www.tkuhn.ch/bel2nanopub/RADQYp1-yuP0Xt2qY1J_TCwufxjKzgtRM3a9ac7FjZphU#_2 http://purl.obolibrary.org/obo/BFO_0000066 http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=10090 http://www.tkuhn.ch/bel2nanopub/RADQYp1-yuP0Xt2qY1J_TCwufxjKzgtRM3a9ac7FjZphU#_2 http://www.w3.org/1999/02/22-rdf-syntax-ns#object http://www.tkuhn.ch/bel2nanopub/RADQYp1-yuP0Xt2qY1J_TCwufxjKzgtRM3a9ac7FjZphU#_1 http://www.tkuhn.ch/bel2nanopub/RADQYp1-yuP0Xt2qY1J_TCwufxjKzgtRM3a9ac7FjZphU#_2 http://www.w3.org/1999/02/22-rdf-syntax-ns#predicate http://www.selventa.com/vocabulary/decreases http://www.tkuhn.ch/bel2nanopub/RADQYp1-yuP0Xt2qY1J_TCwufxjKzgtRM3a9ac7FjZphU#_2 http://www.w3.org/1999/02/22-rdf-syntax-ns#subject http://amigo.geneontology.org/amigo/term/GO:0001696 http://www.tkuhn.ch/bel2nanopub/RADQYp1-yuP0Xt2qY1J_TCwufxjKzgtRM3a9ac7FjZphU#_2 http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.w3.org/1999/02/22-rdf-syntax-ns#Statement http://www.tkuhn.ch/bel2nanopub/RADQYp1-yuP0Xt2qY1J_TCwufxjKzgtRM3a9ac7FjZphU#assertion http://www.w3.org/2000/01/rdf-schema#label bp(GOBP:"gastric acid secretion") -| r(MGI:Anpep) http://www.tkuhn.ch/bel2nanopub/RADQYp1-yuP0Xt2qY1J_TCwufxjKzgtRM3a9ac7FjZphU#provenance http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/description Approximately 61,000 statements. http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/rights Copyright (c) 2011-2012, Selventa. All rights reserved. http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/title BEL Framework Large Corpus Document http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/pav/authoredBy http://www.tkuhn.ch/bel2nanopub/RADQYp1-yuP0Xt2qY1J_TCwufxjKzgtRM3a9ac7FjZphU#_4 http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/pav/version 20131211 http://www.tkuhn.ch/bel2nanopub/RADQYp1-yuP0Xt2qY1J_TCwufxjKzgtRM3a9ac7FjZphU#_3 http://www.w3.org/ns/prov#value Gastrin, a potent stimulator of gastric acid secretion, primarily targets the acid-secreting parietal cells and histamine-secreting enterochromaffin like (ECL) cells in the stomach. Accordingly, gastrin-deficient (GAS-KO) mice have a severe impairment in acid secretion. The aim of this study was to characterize changes in gene expression in GAS-KO mice to identify gastrin-regulated genes and to gain insight into how gastric cell types are regulated by gastrin and acid secretion. Affymetrix microarray analysis of GAS-KO and wild type mice identified numerous differentially expressed transcripts. The results were compared to GAS-KO mice treated with gastrin to identify genes that were gastrin-responsive. Finally, genes that were primarily changed due to gastrin and not hypochlorhydria were identified by comparison to mice that are deficient in both gastrin and cholecystokinin (GAS/CCK-KO), since these mice have restored basal acid secretion. The data were validated by quantitative reverse transcriptase polymerase chain reaction analysis. Interestingly, a number of inflammatory response genes were induced in GAS-KO mice and normalized in GAS/CCK-KO mice, suggesting that they were increased in response to low gastric acid. Moreover, a number of parietal cell transcripts that were down-regulated in GAS-KO mice were similarly restored in GAS/CCK-KO mice, suggesting that parietal cell changes were also primarily associated with hypochlorhydria. In contrast, ECL cell genes that were markedly down-regulated in GAS-KO mice continued to be reduced in GAS/CCK-KO mice, demonstrating that gastrin coordinately regulates a number of ECL cell genes, including several involved in histamine synthesis and secretion. Key words: parietal cell, enterochromaffin-like (ECL) cell, cholecystokinin (CCK), parathyroid hormone-related protein (Pthlh), inflammation. http://www.tkuhn.ch/bel2nanopub/RADQYp1-yuP0Xt2qY1J_TCwufxjKzgtRM3a9ac7FjZphU#_3 http://www.w3.org/ns/prov#wasQuotedFrom http://www.ncbi.nlm.nih.gov/pubmed/17105752 http://www.tkuhn.ch/bel2nanopub/RADQYp1-yuP0Xt2qY1J_TCwufxjKzgtRM3a9ac7FjZphU#_4 http://www.w3.org/2000/01/rdf-schema#label Selventa http://www.tkuhn.ch/bel2nanopub/RADQYp1-yuP0Xt2qY1J_TCwufxjKzgtRM3a9ac7FjZphU#assertion http://www.w3.org/ns/prov#hadPrimarySource http://www.ncbi.nlm.nih.gov/pubmed/17105752 http://www.tkuhn.ch/bel2nanopub/RADQYp1-yuP0Xt2qY1J_TCwufxjKzgtRM3a9ac7FjZphU#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://www.tkuhn.ch/bel2nanopub/RADQYp1-yuP0Xt2qY1J_TCwufxjKzgtRM3a9ac7FjZphU#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://www.tkuhn.ch/bel2nanopub/RADQYp1-yuP0Xt2qY1J_TCwufxjKzgtRM3a9ac7FjZphU#_3 http://www.tkuhn.ch/bel2nanopub/RADQYp1-yuP0Xt2qY1J_TCwufxjKzgtRM3a9ac7FjZphU#pubinfo http://www.tkuhn.ch/bel2nanopub/RADQYp1-yuP0Xt2qY1J_TCwufxjKzgtRM3a9ac7FjZphU http://purl.org/dc/terms/created 2014-07-03T14:33:06.740+02:00 http://www.tkuhn.ch/bel2nanopub/RADQYp1-yuP0Xt2qY1J_TCwufxjKzgtRM3a9ac7FjZphU http://purl.org/pav/createdBy http://orcid.org/0000-0001-6818-334X http://www.tkuhn.ch/bel2nanopub/RADQYp1-yuP0Xt2qY1J_TCwufxjKzgtRM3a9ac7FjZphU http://purl.org/pav/createdBy http://orcid.org/0000-0002-1267-0234