@prefix obo: <http://purl.obolibrary.org/obo/> .
@prefix dce: <http://purl.org/dc/elements/1.1/> .
@prefix pplan: <http://purl.org/net/p-plan#> .
@prefix this: <http://purl.org/np/RACo_xz4b5vLGyjsYQdZZbOfpywHwhvw2E5gp0p8aI8lA> .
@prefix sub: <http://purl.org/np/RACo_xz4b5vLGyjsYQdZZbOfpywHwhvw2E5gp0p8aI8lA#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix sp: <http://purl.org/net/SMARTprotocol#> .

sub:Head {
  this: np:hasAssertion sub:assertion;
    np:hasProvenance sub:provenance;
    np:hasPublicationInfo sub:pubInfo;
    a np:Nanopublication .
}

sub:assertion {
  obo:CHEBI_16991 rdfs:label "DNA" .
  
  obo:OBI_0000439 rdfs:label "agarose gel" .
  
  obo:OBI_0400115 rdfs:label "spectrophotometer" .
  
  sub:_1 rdfs:label "pellet" .
  
  sub:_2 rdfs:label "molecular biology grade water" .
  
  sub:step dce:description "Dissolve pellet in molecular biology grade water and analyze DNA by agarose gel electrophoresis and spectrophotometer for its purity and integrity.";
    sp:hasExperimentalOutput obo:CHEBI_16991;
    a pplan:Step;
    bs:bioSampleUsed obo:CHEBI_16991, sub:_1;
    bs:labEquipmentUsed obo:OBI_0400115;
    bs:reagentUsed obo:OBI_0000439, sub:_2 .
}

sub:provenance {
  sub:assertion pav:createdWith <https://nanotate.bitsfetch.com>;
    prov:generatedAtTime "2021-03-20T05:37:37.743377"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/iannotate> .
}

sub:pubInfo {
  sub:assertion prov:wasDerivedFrom <https://hypothes.is/a/-Kp2MIlnEeuQAjMV80PmeA>,
      authority:3yDt6IlnEeu3Eg8jm5KOGQ, authority:6robKolnEeuyzReiWGxS8Q, authority:CfrS_oloEeuqMS_z19RPyw,
      authority:H48tkIloEeum7Wu6Lhi87A, authority:MfZzsoloEeuW1Ed3UWwXeg .
  
  sub:sig npx:hasAlgorithm "RSA";
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB";
    npx:hasSignature "ZX94AjOsfsOTOyaxkmKTkgx44aCUiPebASvhivRTqnAtpqfz2+V0A8fKoc93HChVqNnOMSwkksVB4RBD6U+A5FRaAfIJRiKcDuIALLlhRi48EaBs18XFD7vYsvuTgNh0N5lKG8V+n45A3i2jenkC9AB7f2lsScHvTWiPS5iVbjI=";
    npx:hasSignatureTarget this: .
  
  this: npx:introduces sub:step;
    prov:generatedAtTime "2021-03-20T05:37:37.743377"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/iannotate> .
}