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http://semanticscience.org/resource/SIO_000139
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tscript(p(HGNC:FOXO3)) -| r(HGNC:CDK4)
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Approximately 61,000 statements.
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Copyright (c) 2011-2012, Selventa. All rights reserved.
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BEL Framework Large Corpus Document
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TABLE 3 Genes involved in proliferation and cell cycle regulation that are repressed by FOXO3.A3.ER and FOXO3.A3.ER.H212R as identified by functional annotation clustering. Affymetrix HG-U133 Plus 2.0 arrays according to the manufacturer?s instructions (Affymetrix, Santa Clara, CA).A Custom CDF Version 11 with Entrez-based gene definitions was used to annotate the arrays (28). Raw fluorescence intensity values were normalized, applying quantile normalization. Differential gene expression was analyzed based on log linear mixed model analysis of variance (29) using the commercial software package SAS JMP7 Genomics, version 3.1 from SAS (SAS Institute, Cary, NC). A false positive rate of 0.05 with Holm correction was taken as level of significance. To be significantly regulated, only genes with a -fold change of 1.5 and a p value less than or equal to the threshold estimated by the Holm multiple testing algorithm, as compared with the empty vector without 4OHT, were considered. Additionally, genes with a significantly different regulation between FOXO3.A3.ER and FOXO3.A3.ER.H212R according to Holm correction and a 2-fold differential regulation between FOXO3.A3.ER and FOXO3.A3.ER.H212R were resorted from the co-regulated group to FOXO3.A3.ER or FOXO3.A3.ER.H212R, respectively.
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Selventa
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2014-07-03T14:33:38.660+02:00
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