http://www.tkuhn.ch/bel2nanopub/RACIsQY2IYd7AUeD7lh4SNyLbi6usBW2pVLFyamic7RQM#Head http://www.tkuhn.ch/bel2nanopub/RACIsQY2IYd7AUeD7lh4SNyLbi6usBW2pVLFyamic7RQM http://www.nanopub.org/nschema#hasAssertion http://www.tkuhn.ch/bel2nanopub/RACIsQY2IYd7AUeD7lh4SNyLbi6usBW2pVLFyamic7RQM#assertion http://www.tkuhn.ch/bel2nanopub/RACIsQY2IYd7AUeD7lh4SNyLbi6usBW2pVLFyamic7RQM http://www.nanopub.org/nschema#hasProvenance http://www.tkuhn.ch/bel2nanopub/RACIsQY2IYd7AUeD7lh4SNyLbi6usBW2pVLFyamic7RQM#provenance http://www.tkuhn.ch/bel2nanopub/RACIsQY2IYd7AUeD7lh4SNyLbi6usBW2pVLFyamic7RQM http://www.nanopub.org/nschema#hasPublicationInfo http://www.tkuhn.ch/bel2nanopub/RACIsQY2IYd7AUeD7lh4SNyLbi6usBW2pVLFyamic7RQM#pubinfo http://www.tkuhn.ch/bel2nanopub/RACIsQY2IYd7AUeD7lh4SNyLbi6usBW2pVLFyamic7RQM http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.nanopub.org/nschema#Nanopublication http://www.tkuhn.ch/bel2nanopub/RACIsQY2IYd7AUeD7lh4SNyLbi6usBW2pVLFyamic7RQM#assertion http://www.tkuhn.ch/bel2nanopub/RACIsQY2IYd7AUeD7lh4SNyLbi6usBW2pVLFyamic7RQM#_1 http://purl.obolibrary.org/obo/RO_0002204 http://www.informatics.jax.org/marker/MGI:88323 http://www.tkuhn.ch/bel2nanopub/RACIsQY2IYd7AUeD7lh4SNyLbi6usBW2pVLFyamic7RQM#_1 http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.ebi.ac.uk/chebi/searchId.do?chebiId=CHEBI_33697 http://www.tkuhn.ch/bel2nanopub/RACIsQY2IYd7AUeD7lh4SNyLbi6usBW2pVLFyamic7RQM#_2 http://purl.obolibrary.org/obo/BFO_0000066 http://purl.obolibrary.org/obo/CL_0000746 http://www.tkuhn.ch/bel2nanopub/RACIsQY2IYd7AUeD7lh4SNyLbi6usBW2pVLFyamic7RQM#_2 http://purl.obolibrary.org/obo/BFO_0000066 http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=10090 http://www.tkuhn.ch/bel2nanopub/RACIsQY2IYd7AUeD7lh4SNyLbi6usBW2pVLFyamic7RQM#_2 http://www.w3.org/1999/02/22-rdf-syntax-ns#object http://www.tkuhn.ch/bel2nanopub/RACIsQY2IYd7AUeD7lh4SNyLbi6usBW2pVLFyamic7RQM#_1 http://www.tkuhn.ch/bel2nanopub/RACIsQY2IYd7AUeD7lh4SNyLbi6usBW2pVLFyamic7RQM#_2 http://www.w3.org/1999/02/22-rdf-syntax-ns#predicate http://www.selventa.com/vocabulary/increases http://www.tkuhn.ch/bel2nanopub/RACIsQY2IYd7AUeD7lh4SNyLbi6usBW2pVLFyamic7RQM#_2 http://www.w3.org/1999/02/22-rdf-syntax-ns#subject http://amigo.geneontology.org/amigo/term/GO:0010002 http://www.tkuhn.ch/bel2nanopub/RACIsQY2IYd7AUeD7lh4SNyLbi6usBW2pVLFyamic7RQM#_2 http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.w3.org/1999/02/22-rdf-syntax-ns#Statement http://www.tkuhn.ch/bel2nanopub/RACIsQY2IYd7AUeD7lh4SNyLbi6usBW2pVLFyamic7RQM#assertion http://www.w3.org/2000/01/rdf-schema#label bp(GOBP:"cardioblast differentiation") -> r(MGI:Cd24a) http://www.tkuhn.ch/bel2nanopub/RACIsQY2IYd7AUeD7lh4SNyLbi6usBW2pVLFyamic7RQM#provenance http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/description Approximately 61,000 statements. http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/rights Copyright (c) 2011-2012, Selventa. All rights reserved. http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/title BEL Framework Large Corpus Document http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/pav/authoredBy http://www.tkuhn.ch/bel2nanopub/RACIsQY2IYd7AUeD7lh4SNyLbi6usBW2pVLFyamic7RQM#_4 http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/pav/version 20131211 http://www.tkuhn.ch/bel2nanopub/RACIsQY2IYd7AUeD7lh4SNyLbi6usBW2pVLFyamic7RQM#_3 http://www.w3.org/ns/prov#value Significant progress has been made in defining pathways that mediate the formation of the mammalian heart. Little is known, however, about the genetic program that directs the differentiation of cardiac myocytes from their precursor cells. A major hindrance to this kind of investigation has been the absence of an appropriate cell culture model of cardiac myocyte differentiation. Recently, a subline of P19 cells (P19CL6) was derived that, following dimethyl sulfoxide (DMSO) treatment, differentiate efficiently over 10 days into spontaneously beating cardiac myocytes. We demonstrate that these cells are indeed cardiac myocytes as they express cell type-specific markers and exhibit electrophysiological properties indicative of cardiac myocytes. The requirement for DMSO stimulation in this paradigm was shown to be limited to the first 4 days, suggesting that critical events in the differentiation process occur over this interval. To uncover relationships among known genes and identify novel genes that mediate cardiac myocyte differentiation, a detailed time course of changes in global gene expression was carried out using cDNA microarrays. In addition to the activation of genes encoding cardiac transcription factors and structural proteins, increases were noted in the expression of multiple known genes and expressed sequence tags (ESTs). Analysis of the former suggested the involvement of a variety of signaling pathways in cardiac myocyte differentiation. The 16 ESTs whose expression was increased during the early, stimulus-dependent phase of cardiac myocyte differentiation may be novel regulators of this process. Thus this first report of large-scale changes in gene expression during cardiac myocyte differentiation has delineated relationships among the expression patterns of known genes and identified a number of novel genes that merit further study. http://www.tkuhn.ch/bel2nanopub/RACIsQY2IYd7AUeD7lh4SNyLbi6usBW2pVLFyamic7RQM#_3 http://www.w3.org/ns/prov#wasQuotedFrom http://www.ncbi.nlm.nih.gov/pubmed/12045295 http://www.tkuhn.ch/bel2nanopub/RACIsQY2IYd7AUeD7lh4SNyLbi6usBW2pVLFyamic7RQM#_4 http://www.w3.org/2000/01/rdf-schema#label Selventa http://www.tkuhn.ch/bel2nanopub/RACIsQY2IYd7AUeD7lh4SNyLbi6usBW2pVLFyamic7RQM#assertion http://www.w3.org/ns/prov#hadPrimarySource http://www.ncbi.nlm.nih.gov/pubmed/12045295 http://www.tkuhn.ch/bel2nanopub/RACIsQY2IYd7AUeD7lh4SNyLbi6usBW2pVLFyamic7RQM#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://www.tkuhn.ch/bel2nanopub/RACIsQY2IYd7AUeD7lh4SNyLbi6usBW2pVLFyamic7RQM#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://www.tkuhn.ch/bel2nanopub/RACIsQY2IYd7AUeD7lh4SNyLbi6usBW2pVLFyamic7RQM#_3 http://www.tkuhn.ch/bel2nanopub/RACIsQY2IYd7AUeD7lh4SNyLbi6usBW2pVLFyamic7RQM#pubinfo http://www.tkuhn.ch/bel2nanopub/RACIsQY2IYd7AUeD7lh4SNyLbi6usBW2pVLFyamic7RQM http://purl.org/dc/terms/created 2014-07-03T14:31:49.797+02:00 http://www.tkuhn.ch/bel2nanopub/RACIsQY2IYd7AUeD7lh4SNyLbi6usBW2pVLFyamic7RQM http://purl.org/pav/createdBy http://orcid.org/0000-0001-6818-334X http://www.tkuhn.ch/bel2nanopub/RACIsQY2IYd7AUeD7lh4SNyLbi6usBW2pVLFyamic7RQM http://purl.org/pav/createdBy http://orcid.org/0000-0002-1267-0234