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All rights reserved. http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/title BEL Framework Large Corpus Document http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/pav/authoredBy http://www.tkuhn.ch/bel2nanopub/RACEnp0V5A5UnRIUTxaeR_PLdur-OZMcnXiK4HD4v1ll4#_4 http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/pav/version 20131211 http://www.tkuhn.ch/bel2nanopub/RACEnp0V5A5UnRIUTxaeR_PLdur-OZMcnXiK4HD4v1ll4#_3 http://www.w3.org/ns/prov#value Gastrin, a potent stimulator of gastric acid secretion, primarily targets the acid-secreting parietal cells and histamine-secreting enterochromaffin like (ECL) cells in the stomach. Accordingly, gastrin-deficient (GAS-KO) mice have a severe impairment in acid secretion. The aim of this study was to characterize changes in gene expression in GAS-KO mice to identify gastrin-regulated genes and to gain insight into how gastric cell types are regulated by gastrin and acid secretion. Affymetrix microarray analysis of GAS-KO and wild type mice identified numerous differentially expressed transcripts. The results were compared to GAS-KO mice treated with gastrin to identify genes that were gastrin-responsive. Finally, genes that were primarily changed due to gastrin and not hypochlorhydria were identified by comparison to mice that are deficient in both gastrin and cholecystokinin (GAS/CCK-KO), since these mice have restored basal acid secretion. The data were validated by quantitative reverse transcriptase polymerase chain reaction analysis. Interestingly, a number of inflammatory response genes were induced in GAS-KO mice and normalized in GAS/CCK-KO mice, suggesting that they were increased in response to low gastric acid. Moreover, a number of parietal cell transcripts that were down-regulated in GAS-KO mice were similarly restored in GAS/CCK-KO mice, suggesting that parietal cell changes were also primarily associated with hypochlorhydria. In contrast, ECL cell genes that were markedly down-regulated in GAS-KO mice continued to be reduced in GAS/CCK-KO mice, demonstrating that gastrin coordinately regulates a number of ECL cell genes, including several involved in histamine synthesis and secretion. Key words: parietal cell, enterochromaffin-like (ECL) cell, cholecystokinin (CCK), parathyroid hormone-related protein (Pthlh), inflammation. http://www.tkuhn.ch/bel2nanopub/RACEnp0V5A5UnRIUTxaeR_PLdur-OZMcnXiK4HD4v1ll4#_3 http://www.w3.org/ns/prov#wasQuotedFrom http://www.ncbi.nlm.nih.gov/pubmed/17105752 http://www.tkuhn.ch/bel2nanopub/RACEnp0V5A5UnRIUTxaeR_PLdur-OZMcnXiK4HD4v1ll4#_4 http://www.w3.org/2000/01/rdf-schema#label Selventa http://www.tkuhn.ch/bel2nanopub/RACEnp0V5A5UnRIUTxaeR_PLdur-OZMcnXiK4HD4v1ll4#assertion http://www.w3.org/ns/prov#hadPrimarySource http://www.ncbi.nlm.nih.gov/pubmed/17105752 http://www.tkuhn.ch/bel2nanopub/RACEnp0V5A5UnRIUTxaeR_PLdur-OZMcnXiK4HD4v1ll4#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://www.tkuhn.ch/bel2nanopub/RACEnp0V5A5UnRIUTxaeR_PLdur-OZMcnXiK4HD4v1ll4#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://www.tkuhn.ch/bel2nanopub/RACEnp0V5A5UnRIUTxaeR_PLdur-OZMcnXiK4HD4v1ll4#_3 http://www.tkuhn.ch/bel2nanopub/RACEnp0V5A5UnRIUTxaeR_PLdur-OZMcnXiK4HD4v1ll4#pubinfo http://www.tkuhn.ch/bel2nanopub/RACEnp0V5A5UnRIUTxaeR_PLdur-OZMcnXiK4HD4v1ll4 http://purl.org/dc/terms/created 2014-07-03T14:33:06.772+02:00 http://www.tkuhn.ch/bel2nanopub/RACEnp0V5A5UnRIUTxaeR_PLdur-OZMcnXiK4HD4v1ll4 http://purl.org/pav/createdBy http://orcid.org/0000-0001-6818-334X http://www.tkuhn.ch/bel2nanopub/RACEnp0V5A5UnRIUTxaeR_PLdur-OZMcnXiK4HD4v1ll4 http://purl.org/pav/createdBy http://orcid.org/0000-0002-1267-0234