http://www.tkuhn.ch/bel2nanopub/RACAsVRVL5O-wkzRswvBBRV_l1CAbW7NPcy5tlKx61x3A#Head http://www.tkuhn.ch/bel2nanopub/RACAsVRVL5O-wkzRswvBBRV_l1CAbW7NPcy5tlKx61x3A http://www.nanopub.org/nschema#hasAssertion http://www.tkuhn.ch/bel2nanopub/RACAsVRVL5O-wkzRswvBBRV_l1CAbW7NPcy5tlKx61x3A#assertion http://www.tkuhn.ch/bel2nanopub/RACAsVRVL5O-wkzRswvBBRV_l1CAbW7NPcy5tlKx61x3A http://www.nanopub.org/nschema#hasProvenance http://www.tkuhn.ch/bel2nanopub/RACAsVRVL5O-wkzRswvBBRV_l1CAbW7NPcy5tlKx61x3A#provenance http://www.tkuhn.ch/bel2nanopub/RACAsVRVL5O-wkzRswvBBRV_l1CAbW7NPcy5tlKx61x3A http://www.nanopub.org/nschema#hasPublicationInfo http://www.tkuhn.ch/bel2nanopub/RACAsVRVL5O-wkzRswvBBRV_l1CAbW7NPcy5tlKx61x3A#pubinfo http://www.tkuhn.ch/bel2nanopub/RACAsVRVL5O-wkzRswvBBRV_l1CAbW7NPcy5tlKx61x3A http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.nanopub.org/nschema#Nanopublication http://www.tkuhn.ch/bel2nanopub/RACAsVRVL5O-wkzRswvBBRV_l1CAbW7NPcy5tlKx61x3A#assertion http://www.tkuhn.ch/bel2nanopub/RACAsVRVL5O-wkzRswvBBRV_l1CAbW7NPcy5tlKx61x3A#_1 http://purl.obolibrary.org/obo/RO_0002204 http://rgd.mcw.edu/rgdweb/report/gene/main.html?id=621178 http://www.tkuhn.ch/bel2nanopub/RACAsVRVL5O-wkzRswvBBRV_l1CAbW7NPcy5tlKx61x3A#_1 http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.ebi.ac.uk/chebi/searchId.do?chebiId=CHEBI_33697 http://www.tkuhn.ch/bel2nanopub/RACAsVRVL5O-wkzRswvBBRV_l1CAbW7NPcy5tlKx61x3A#_2 http://purl.obolibrary.org/obo/BFO_0000066 http://purl.obolibrary.org/obo/CL_0000540 http://www.tkuhn.ch/bel2nanopub/RACAsVRVL5O-wkzRswvBBRV_l1CAbW7NPcy5tlKx61x3A#_2 http://purl.obolibrary.org/obo/BFO_0000066 http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=10116 http://www.tkuhn.ch/bel2nanopub/RACAsVRVL5O-wkzRswvBBRV_l1CAbW7NPcy5tlKx61x3A#_2 http://www.w3.org/1999/02/22-rdf-syntax-ns#object http://www.tkuhn.ch/bel2nanopub/RACAsVRVL5O-wkzRswvBBRV_l1CAbW7NPcy5tlKx61x3A#_1 http://www.tkuhn.ch/bel2nanopub/RACAsVRVL5O-wkzRswvBBRV_l1CAbW7NPcy5tlKx61x3A#_2 http://www.w3.org/1999/02/22-rdf-syntax-ns#predicate http://www.selventa.com/vocabulary/increases http://www.tkuhn.ch/bel2nanopub/RACAsVRVL5O-wkzRswvBBRV_l1CAbW7NPcy5tlKx61x3A#_2 http://www.w3.org/1999/02/22-rdf-syntax-ns#subject http://resource.belframework.org/belframework/20131211/namespace/selventa-legacy-diseases/burn%20injury http://www.tkuhn.ch/bel2nanopub/RACAsVRVL5O-wkzRswvBBRV_l1CAbW7NPcy5tlKx61x3A#_2 http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.w3.org/1999/02/22-rdf-syntax-ns#Statement http://www.tkuhn.ch/bel2nanopub/RACAsVRVL5O-wkzRswvBBRV_l1CAbW7NPcy5tlKx61x3A#assertion http://www.w3.org/2000/01/rdf-schema#label path(SDIS:"burn injury") -> r(RGD:Rpl10) http://www.tkuhn.ch/bel2nanopub/RACAsVRVL5O-wkzRswvBBRV_l1CAbW7NPcy5tlKx61x3A#provenance http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/description Approximately 61,000 statements. http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/rights Copyright (c) 2011-2012, Selventa. All rights reserved. http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/title BEL Framework Large Corpus Document http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/pav/authoredBy http://www.tkuhn.ch/bel2nanopub/RACAsVRVL5O-wkzRswvBBRV_l1CAbW7NPcy5tlKx61x3A#_4 http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/pav/version 20131211 http://www.tkuhn.ch/bel2nanopub/RACAsVRVL5O-wkzRswvBBRV_l1CAbW7NPcy5tlKx61x3A#_3 http://www.w3.org/ns/prov#value Three experimental models of axonal injuries in adult rat spinal motoneurons were established to investigate changes of gene expression in response to such injuries. We took advantage of cDNA microarray analysis to determine the differential expression of genes in injured motoneurons following distal axotomy or root avulsion in the absence or presence of BDNF. The major finding was that, in response to proximal axonal injury (avulsion), expression of genes that are known to facilitate neuronal survival and axonal regeneration (e.g., IGFRII, PI3K, IGFBP-6, GSTs, GalR2) were down-regulated; but following treatment with BDNF they were up-regulated. In addition, the expression of genes known to be involved in apoptosis and DNA damage (e.g., ANX5, TS, ALR) were down-regulated in BDNF-treated animals with avulsion. Furthermore, many functional families of genes previously shown to play roles in the pathophysiology of axonal injury, including SNAP-25A, SV2B, Ras-related ras3a/4b, ERK1/2, 14-3-3 proteins, proteasome proteins, oncogenes, GAP-43, and NMDAR1, were altered after either distal axotomy or avulsion injury. Some of the changes in gene expression, including Lim-2, FRAG1, GlaR2, GSTs, ALR, TS, ANX3/5, and nhe1/2, are first reported here in injured motoneurons. The differential expression of genes identified by the expression arrays was confirmed by gene-specific RT-PCR for eight genes (GAP-43, IGFR II, Lim-2, MIF, NDAP1, TS, PCC3, and FRAG1) and by in situ hybridization for Lim-2. These results suggest that abnormal regulation of particular biochemical pathways may induce motoneuron death after ventral root avulsion in adult animals. This study presents an approach for selecting specific genes and their products that may be involved in motoneuron degeneration following axonal injuries. http://www.tkuhn.ch/bel2nanopub/RACAsVRVL5O-wkzRswvBBRV_l1CAbW7NPcy5tlKx61x3A#_3 http://www.w3.org/ns/prov#wasQuotedFrom http://www.ncbi.nlm.nih.gov/pubmed/16460709 http://www.tkuhn.ch/bel2nanopub/RACAsVRVL5O-wkzRswvBBRV_l1CAbW7NPcy5tlKx61x3A#_4 http://www.w3.org/2000/01/rdf-schema#label Selventa http://www.tkuhn.ch/bel2nanopub/RACAsVRVL5O-wkzRswvBBRV_l1CAbW7NPcy5tlKx61x3A#assertion http://www.w3.org/ns/prov#hadPrimarySource http://www.ncbi.nlm.nih.gov/pubmed/16460709 http://www.tkuhn.ch/bel2nanopub/RACAsVRVL5O-wkzRswvBBRV_l1CAbW7NPcy5tlKx61x3A#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://www.tkuhn.ch/bel2nanopub/RACAsVRVL5O-wkzRswvBBRV_l1CAbW7NPcy5tlKx61x3A#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://www.tkuhn.ch/bel2nanopub/RACAsVRVL5O-wkzRswvBBRV_l1CAbW7NPcy5tlKx61x3A#_3 http://www.tkuhn.ch/bel2nanopub/RACAsVRVL5O-wkzRswvBBRV_l1CAbW7NPcy5tlKx61x3A#pubinfo http://www.tkuhn.ch/bel2nanopub/RACAsVRVL5O-wkzRswvBBRV_l1CAbW7NPcy5tlKx61x3A http://purl.org/dc/terms/created 2014-07-03T14:32:55.980+02:00 http://www.tkuhn.ch/bel2nanopub/RACAsVRVL5O-wkzRswvBBRV_l1CAbW7NPcy5tlKx61x3A http://purl.org/pav/createdBy http://orcid.org/0000-0001-6818-334X http://www.tkuhn.ch/bel2nanopub/RACAsVRVL5O-wkzRswvBBRV_l1CAbW7NPcy5tlKx61x3A http://purl.org/pav/createdBy http://orcid.org/0000-0002-1267-0234