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p(MGI:Thbs4) -> bp(GO:"myoblast differentiation")
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Approximately 61,000 statements.
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Copyright (c) 2011-2012, Selventa. All rights reserved.
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BEL Framework Large Corpus Document
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A previous in vitro study demonstrated that myoblasts adhere to a peptide corresponding to the cell-binding carboxyl-terminal end of TSP-4 (Adams and Lawler, 1994). Accordingly, to determine whether TSP-4 can promote myogenic differentiation we carried out coculture experiments with primary myoblasts from newborn mice and TSP-4-expressing COS cells (COS-TSP- 4; Arber and Caroni, 1995). The latter secrete substantial amounts of TSP-4, which was shown in a previous study to efficiently promote neurite outgrowth (Arber and Caroni, 1995). COS-TSP-4 or naive COS cells were plated at low density on laminin-coated culture dishes, and hind limb muscle-derived cells were added 4-5 h later. After 2 d in growth-promoting medium the cultures were switched to differentiation medium, and myotube formation was scored 1 and 2 d later. As shown in Fig. 10, under these experimental conditions, myogenic differentiation and myotube formation were markedly potentiated in the presence of the COS-TSP-4 cells. Muscle-derived cells, which were noticably smaller and thus easily distinguishable from cocultured COS cells, could be detected in comparable amounts in the two types of cultures. However, in the absence of TSP-4 these cells only expressed low levels of muscle-specifi alpha-actinin and only rarely fused to myotubes (Fig. 10).
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Selventa
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2014-07-03T14:30:59.051+02:00
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