. . . . . . . . . . . . . . . . . . . . . . "p(HGNC:SOCS3) -| (p(SFAM:\"IFNA Family\") -> tscript(p(HGNC:STAT1)))" . "p(SFAM:\"IFNA Family\") -> tscript(p(HGNC:STAT1))" . "Approximately 61,000 statements." . "Copyright (c) 2011-2012, Selventa. All rights reserved." . "BEL Framework Large Corpus Document" . . "20131211" . "We have previously shown that in CTCL cells transfected with a dominant negative Stat3, the absence of endogenous SOCS-3 coincides with increased IFNalpha signaling.19 To provide direct evidence that SOCS-3 does indeed modulate the response to this cytokine, we examined IFNalpha responses in (i) wt CTCL, (ii) CTCL cells expressing inducible SOCS-3, and (iii) CTCL cells expressing the dominant negative Stat3 (Stat3D), which inhibits the endogenous SOCS-3 expression.19 In agreement with the difference seen previously between SOCS-3hi and SOCS-3lo cells,19 expression of SOCS-3 caused a marked reduction in IFNalpha-induced phosphorylation of both Stat1 and Stat3 (Figure 4a lanes 6 and 8, respectively), whereas induction of the nonactive SOCS-3 mutant had no effect (lanes 10 and 12). Figure 4 legend: SOCS-3 expression inhibits Stat activation induced by IFNalpha and IL-2, but not IL-4. " . . "Selventa" . . . . "2014-07-03T14:32:37.169+02:00"^^ . . .