sub:provenance {
  beldoc: dce:description "Approximately 61,000 statements." ;
    dce:rights "Copyright (c) 2011-2012, Selventa. All rights reserved." ;
    dce:title "BEL Framework Large Corpus Document" ;
    pav:authoredBy sub:_4 ;
    pav:version "20131211" .
  sub:_3 prov:value "Human microvascular endothelial cells from lung (HMVEC-Lung, HMVEC-L) and cardiac (HMVEC-Cardiac, HMVEC-C) cells were cultured and exposed to hypoxia (1% O2, 5% CO2, and 94% N2) on 6 separate occasions, and total RNA was extracted. Experimental samples (n = 3) were formed by randomly pooling RNA from 2 independent experiments. This process was repeated at each of 4 experimental time points yielding 12 separate arrays for lung and cardiac experiments. Affymetrix GeneChip Human Genome U133A chips, which allow the simultaneous analysis of 22,283 gene transcripts, were used. * Genstruct R-pipeline analysis of GSE11341 conducted Nov 2010 by DD.  Cutoffs for curation applied by AV were FC>1.5 and adj. p<.01. The 48h treatment timepoint was selected for curation because, as the paper reports, \\\"An incremental increase in the expression of 2 well-characterized hypoxia-responsive marker genes, namely VEGF-A and solute carrier family 2 member 1 [SLC2A1 (previously called GLUT1)], was measured by RT-PCR.\\\"" ;
    prov:wasQuotedFrom pubmed:18469115 .
  sub:_4 rdfs:label "Selventa" .
  sub:assertion prov:hadPrimarySource pubmed:18469115 ;
    prov:wasDerivedFrom beldoc: , sub:_3 .
}