http://www.tkuhn.ch/bel2nanopub/RAASIi8qHDuFcdTxT88uedFIEyYXGw1SVpsVIlnxIsOnI#Head http://www.tkuhn.ch/bel2nanopub/RAASIi8qHDuFcdTxT88uedFIEyYXGw1SVpsVIlnxIsOnI http://www.nanopub.org/nschema#hasAssertion http://www.tkuhn.ch/bel2nanopub/RAASIi8qHDuFcdTxT88uedFIEyYXGw1SVpsVIlnxIsOnI#assertion http://www.tkuhn.ch/bel2nanopub/RAASIi8qHDuFcdTxT88uedFIEyYXGw1SVpsVIlnxIsOnI http://www.nanopub.org/nschema#hasProvenance http://www.tkuhn.ch/bel2nanopub/RAASIi8qHDuFcdTxT88uedFIEyYXGw1SVpsVIlnxIsOnI#provenance http://www.tkuhn.ch/bel2nanopub/RAASIi8qHDuFcdTxT88uedFIEyYXGw1SVpsVIlnxIsOnI http://www.nanopub.org/nschema#hasPublicationInfo http://www.tkuhn.ch/bel2nanopub/RAASIi8qHDuFcdTxT88uedFIEyYXGw1SVpsVIlnxIsOnI#pubinfo http://www.tkuhn.ch/bel2nanopub/RAASIi8qHDuFcdTxT88uedFIEyYXGw1SVpsVIlnxIsOnI http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.nanopub.org/nschema#Nanopublication http://www.tkuhn.ch/bel2nanopub/RAASIi8qHDuFcdTxT88uedFIEyYXGw1SVpsVIlnxIsOnI#assertion http://www.tkuhn.ch/bel2nanopub/RAASIi8qHDuFcdTxT88uedFIEyYXGw1SVpsVIlnxIsOnI#_1 http://purl.obolibrary.org/obo/RO_0002204 http://www.genenames.org/cgi-bin/gene_symbol_report?hgnc_id=7995 http://www.tkuhn.ch/bel2nanopub/RAASIi8qHDuFcdTxT88uedFIEyYXGw1SVpsVIlnxIsOnI#_1 http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.ebi.ac.uk/chebi/searchId.do?chebiId=CHEBI_33697 http://www.tkuhn.ch/bel2nanopub/RAASIi8qHDuFcdTxT88uedFIEyYXGw1SVpsVIlnxIsOnI#_2 http://purl.obolibrary.org/obo/BFO_0000066 http://purl.obolibrary.org/obo/CL_0000127 http://www.tkuhn.ch/bel2nanopub/RAASIi8qHDuFcdTxT88uedFIEyYXGw1SVpsVIlnxIsOnI#_2 http://purl.obolibrary.org/obo/BFO_0000066 http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=9606 http://www.tkuhn.ch/bel2nanopub/RAASIi8qHDuFcdTxT88uedFIEyYXGw1SVpsVIlnxIsOnI#_2 http://www.w3.org/1999/02/22-rdf-syntax-ns#object http://www.tkuhn.ch/bel2nanopub/RAASIi8qHDuFcdTxT88uedFIEyYXGw1SVpsVIlnxIsOnI#_1 http://www.tkuhn.ch/bel2nanopub/RAASIi8qHDuFcdTxT88uedFIEyYXGw1SVpsVIlnxIsOnI#_2 http://www.w3.org/1999/02/22-rdf-syntax-ns#predicate http://www.selventa.com/vocabulary/increases http://www.tkuhn.ch/bel2nanopub/RAASIi8qHDuFcdTxT88uedFIEyYXGw1SVpsVIlnxIsOnI#_2 http://www.w3.org/1999/02/22-rdf-syntax-ns#subject http://amigo.geneontology.org/amigo/term/GO:0001666 http://www.tkuhn.ch/bel2nanopub/RAASIi8qHDuFcdTxT88uedFIEyYXGw1SVpsVIlnxIsOnI#_2 http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.w3.org/1999/02/22-rdf-syntax-ns#Statement http://www.tkuhn.ch/bel2nanopub/RAASIi8qHDuFcdTxT88uedFIEyYXGw1SVpsVIlnxIsOnI#assertion http://www.w3.org/2000/01/rdf-schema#label bp(GOBP:"response to hypoxia") -> r(HGNC:NRD1) http://www.tkuhn.ch/bel2nanopub/RAASIi8qHDuFcdTxT88uedFIEyYXGw1SVpsVIlnxIsOnI#provenance http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/description Approximately 61,000 statements. http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/rights Copyright (c) 2011-2012, Selventa. All rights reserved. http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/title BEL Framework Large Corpus Document http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/pav/authoredBy http://www.tkuhn.ch/bel2nanopub/RAASIi8qHDuFcdTxT88uedFIEyYXGw1SVpsVIlnxIsOnI#_4 http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/pav/version 20131211 http://www.tkuhn.ch/bel2nanopub/RAASIi8qHDuFcdTxT88uedFIEyYXGw1SVpsVIlnxIsOnI#_3 http://www.w3.org/ns/prov#value Oxygen is vital for the development and survival of mammals. In response to hypoxia, the brain initiates numerous adaptive responses at the organ level as well as at the molecular and cellular levels, including the alteration of gene expression. Astrocytes play critical roles in the proper functioning of the brain; thus the manner in which astrocytes respond to hypoxia is likely important in determining the outcome of brain hypoxia. Here, we used microarray gene expression profiling and data-analysis algorithms to identify and analyze hypoxia-responsive genes in primary human astrocytes. We also compared gene expression patterns in astrocytes with those in human HeLa cells and pulmonary artery endothelial cells (ECs). Remarkably, in astrocytes, five times as many genes were induced as suppressed, whereas in HeLa and pulmonary ECs, as many as or more genes were suppressed than induced. More genes encoding hypoxia-inducible functions, such as glycolytic enzymes and angiogenic growth factors, were strongly induced in astrocytes compared with HeLa cells. Furthermore, gene ontology and computational algorithms revealed that many target genes of the EGF and insulin signaling pathways and the transcriptional regulators Myc, Jun, and p53 were selectively altered by hypoxia in astrocytes. Indeed, Western blot analysis confirmed that two major signal transducers mediating insulin and EGF action, Akt and MEK1/2, were activated by hypoxia in astrocytes. These results provide a global view of the signaling and regulatory network mediating oxygen regulation in human astrocytes. http://www.tkuhn.ch/bel2nanopub/RAASIi8qHDuFcdTxT88uedFIEyYXGw1SVpsVIlnxIsOnI#_3 http://www.w3.org/ns/prov#wasQuotedFrom http://www.ncbi.nlm.nih.gov/pubmed/16507782 http://www.tkuhn.ch/bel2nanopub/RAASIi8qHDuFcdTxT88uedFIEyYXGw1SVpsVIlnxIsOnI#_4 http://www.w3.org/2000/01/rdf-schema#label Selventa http://www.tkuhn.ch/bel2nanopub/RAASIi8qHDuFcdTxT88uedFIEyYXGw1SVpsVIlnxIsOnI#assertion http://www.w3.org/ns/prov#hadPrimarySource http://www.ncbi.nlm.nih.gov/pubmed/16507782 http://www.tkuhn.ch/bel2nanopub/RAASIi8qHDuFcdTxT88uedFIEyYXGw1SVpsVIlnxIsOnI#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://www.tkuhn.ch/bel2nanopub/RAASIi8qHDuFcdTxT88uedFIEyYXGw1SVpsVIlnxIsOnI#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://www.tkuhn.ch/bel2nanopub/RAASIi8qHDuFcdTxT88uedFIEyYXGw1SVpsVIlnxIsOnI#_3 http://www.tkuhn.ch/bel2nanopub/RAASIi8qHDuFcdTxT88uedFIEyYXGw1SVpsVIlnxIsOnI#pubinfo http://www.tkuhn.ch/bel2nanopub/RAASIi8qHDuFcdTxT88uedFIEyYXGw1SVpsVIlnxIsOnI http://purl.org/dc/terms/created 2014-07-03T14:32:56.725+02:00 http://www.tkuhn.ch/bel2nanopub/RAASIi8qHDuFcdTxT88uedFIEyYXGw1SVpsVIlnxIsOnI http://purl.org/pav/createdBy http://orcid.org/0000-0001-6818-334X http://www.tkuhn.ch/bel2nanopub/RAASIi8qHDuFcdTxT88uedFIEyYXGw1SVpsVIlnxIsOnI http://purl.org/pav/createdBy http://orcid.org/0000-0002-1267-0234