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All rights reserved. http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/title BEL Framework Large Corpus Document http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/pav/authoredBy http://www.tkuhn.ch/bel2nanopub/RAAIC9gQsRt45DP3e1lnu0jm_gubI5aUX36qBTVciyOqk#_5 http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/pav/version 1.4 http://www.tkuhn.ch/bel2nanopub/RAAIC9gQsRt45DP3e1lnu0jm_gubI5aUX36qBTVciyOqk#_4 http://www.w3.org/ns/prov#value Blnk and Ifit1 showed no increase in either the HOXD13- or HOXA9-expressing cells. Given that Blnk had increased expression after transient HOXD13 expression, it is likely that the lack of a response may be due to low HOXD13 levels in this population of HOXD13 stable-expressing cells. Since the SQ RT-PCR assay is not as useful for detecting changes in expression of genes that are initially highly expressed in the EGFP-only cells, we did not test the expression of Fstl and Igfbp4. Ngef and Casp8ap2 had lower expression levels in the HOXA13/EGFP-expressing cells (Ngef, Fig. 2; Casp8ap2 decreased 1.3-fold, not shown). HOXD13- and HOXA9-expressing cells also had reduced expression of Ngef and Casp8ap2 (Fig. 7B) by a magnitude consistent with that observed by microarray for the HOXA13/EGFP-expressing cells (Table 3). Thus, these results demonstrate that group 13 HOX proteins were capable of upregulating a subset of the genes that HOXA9 could not, implying a paralog-specific regulatory effect. 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