http://www.tkuhn.ch/bel2nanopub/RA8hglCFNv_9ntjaGHJosMwPIMOZl1VFh1zAje9EhDpQM#Head http://www.tkuhn.ch/bel2nanopub/RA8hglCFNv_9ntjaGHJosMwPIMOZl1VFh1zAje9EhDpQM http://www.nanopub.org/nschema#hasAssertion http://www.tkuhn.ch/bel2nanopub/RA8hglCFNv_9ntjaGHJosMwPIMOZl1VFh1zAje9EhDpQM#assertion http://www.tkuhn.ch/bel2nanopub/RA8hglCFNv_9ntjaGHJosMwPIMOZl1VFh1zAje9EhDpQM http://www.nanopub.org/nschema#hasProvenance http://www.tkuhn.ch/bel2nanopub/RA8hglCFNv_9ntjaGHJosMwPIMOZl1VFh1zAje9EhDpQM#provenance http://www.tkuhn.ch/bel2nanopub/RA8hglCFNv_9ntjaGHJosMwPIMOZl1VFh1zAje9EhDpQM http://www.nanopub.org/nschema#hasPublicationInfo http://www.tkuhn.ch/bel2nanopub/RA8hglCFNv_9ntjaGHJosMwPIMOZl1VFh1zAje9EhDpQM#pubinfo http://www.tkuhn.ch/bel2nanopub/RA8hglCFNv_9ntjaGHJosMwPIMOZl1VFh1zAje9EhDpQM http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.nanopub.org/nschema#Nanopublication http://www.tkuhn.ch/bel2nanopub/RA8hglCFNv_9ntjaGHJosMwPIMOZl1VFh1zAje9EhDpQM#assertion http://www.tkuhn.ch/bel2nanopub/RA8hglCFNv_9ntjaGHJosMwPIMOZl1VFh1zAje9EhDpQM#_1 http://purl.obolibrary.org/obo/RO_0002204 http://rgd.mcw.edu/rgdweb/report/gene/main.html?id=621043 http://www.tkuhn.ch/bel2nanopub/RA8hglCFNv_9ntjaGHJosMwPIMOZl1VFh1zAje9EhDpQM#_1 http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.ebi.ac.uk/chebi/searchId.do?chebiId=CHEBI_33697 http://www.tkuhn.ch/bel2nanopub/RA8hglCFNv_9ntjaGHJosMwPIMOZl1VFh1zAje9EhDpQM#_2 http://purl.obolibrary.org/obo/BFO_0000066 http://purl.bioontology.org/ontology/MSH/D008099 http://www.tkuhn.ch/bel2nanopub/RA8hglCFNv_9ntjaGHJosMwPIMOZl1VFh1zAje9EhDpQM#_2 http://purl.obolibrary.org/obo/BFO_0000066 http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=10116 http://www.tkuhn.ch/bel2nanopub/RA8hglCFNv_9ntjaGHJosMwPIMOZl1VFh1zAje9EhDpQM#_2 http://www.w3.org/1999/02/22-rdf-syntax-ns#object http://www.tkuhn.ch/bel2nanopub/RA8hglCFNv_9ntjaGHJosMwPIMOZl1VFh1zAje9EhDpQM#_1 http://www.tkuhn.ch/bel2nanopub/RA8hglCFNv_9ntjaGHJosMwPIMOZl1VFh1zAje9EhDpQM#_2 http://www.w3.org/1999/02/22-rdf-syntax-ns#predicate http://www.selventa.com/vocabulary/increases http://www.tkuhn.ch/bel2nanopub/RA8hglCFNv_9ntjaGHJosMwPIMOZl1VFh1zAje9EhDpQM#_2 http://www.w3.org/1999/02/22-rdf-syntax-ns#subject http://amigo.geneontology.org/amigo/term/GO:0016280 http://www.tkuhn.ch/bel2nanopub/RA8hglCFNv_9ntjaGHJosMwPIMOZl1VFh1zAje9EhDpQM#_2 http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.w3.org/1999/02/22-rdf-syntax-ns#Statement http://www.tkuhn.ch/bel2nanopub/RA8hglCFNv_9ntjaGHJosMwPIMOZl1VFh1zAje9EhDpQM#assertion http://www.w3.org/2000/01/rdf-schema#label bp(GO:aging) -> r(RGD:Rps25) http://www.tkuhn.ch/bel2nanopub/RA8hglCFNv_9ntjaGHJosMwPIMOZl1VFh1zAje9EhDpQM#provenance http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/description Approximately 61,000 statements. http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/rights Copyright (c) 2011-2012, Selventa. All rights reserved. http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/title BEL Framework Large Corpus Document http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/pav/authoredBy http://www.tkuhn.ch/bel2nanopub/RA8hglCFNv_9ntjaGHJosMwPIMOZl1VFh1zAje9EhDpQM#_4 http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/pav/version 1.4 http://www.tkuhn.ch/bel2nanopub/RA8hglCFNv_9ntjaGHJosMwPIMOZl1VFh1zAje9EhDpQM#_3 http://www.w3.org/ns/prov#value BACKGROUND: It appears that consistent changes in the levels of activity of a small cohort of genes (probably mammalian cells during ageing. Identification of such genes could provide valuable insights into the ageing process. OBJECTIVE: We have studied age-related changes in gene expression profiles in the rat liver. One of the genes which exhibited clear and consistent increases in expression is characterised. METHODS: Analysis of the gene expression profile was carried out using a combination of an optimised form of differential display and single-strand conformational polymorphism (SSCP) gel analysis. Gene expression levels were quantified by Northern blot analysis. The gene's identity was determined by comparing its nucleotide sequence to DNA databases. RESULTS: During this investigation we observed one gene which exhibited an increase in expression in livers from young adult (6 months) to old adult (24 months) rats. The differential expression of this gene was confirmed by SSCP gel analysis and Northern blotting. Densitometry of the latter indicated that expression increased by 165% with age. Characterisation of the isolated polymerase chain reaction fragment demonstrated it to code for the ribosomal protein S25 (RPS25). CONCLUSIONS: This increase in RPS25 expression is further evidence that the composition of ribosomes may alter with age and as a result could have significant effects on protein synthesis. http://www.tkuhn.ch/bel2nanopub/RA8hglCFNv_9ntjaGHJosMwPIMOZl1VFh1zAje9EhDpQM#_3 http://www.w3.org/ns/prov#wasQuotedFrom http://www.ncbi.nlm.nih.gov/pubmed/12393952 http://www.tkuhn.ch/bel2nanopub/RA8hglCFNv_9ntjaGHJosMwPIMOZl1VFh1zAje9EhDpQM#_4 http://www.w3.org/2000/01/rdf-schema#label Selventa http://www.tkuhn.ch/bel2nanopub/RA8hglCFNv_9ntjaGHJosMwPIMOZl1VFh1zAje9EhDpQM#assertion http://www.w3.org/ns/prov#hadPrimarySource http://www.ncbi.nlm.nih.gov/pubmed/12393952 http://www.tkuhn.ch/bel2nanopub/RA8hglCFNv_9ntjaGHJosMwPIMOZl1VFh1zAje9EhDpQM#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://www.tkuhn.ch/bel2nanopub/RA8hglCFNv_9ntjaGHJosMwPIMOZl1VFh1zAje9EhDpQM#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://www.tkuhn.ch/bel2nanopub/RA8hglCFNv_9ntjaGHJosMwPIMOZl1VFh1zAje9EhDpQM#_3 http://www.tkuhn.ch/bel2nanopub/RA8hglCFNv_9ntjaGHJosMwPIMOZl1VFh1zAje9EhDpQM#pubinfo http://www.tkuhn.ch/bel2nanopub/RA8hglCFNv_9ntjaGHJosMwPIMOZl1VFh1zAje9EhDpQM http://purl.org/dc/terms/created 2014-07-03T14:30:06.634+02:00 http://www.tkuhn.ch/bel2nanopub/RA8hglCFNv_9ntjaGHJosMwPIMOZl1VFh1zAje9EhDpQM http://purl.org/pav/createdBy http://orcid.org/0000-0001-6818-334X http://www.tkuhn.ch/bel2nanopub/RA8hglCFNv_9ntjaGHJosMwPIMOZl1VFh1zAje9EhDpQM http://purl.org/pav/createdBy http://orcid.org/0000-0002-1267-0234