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@prefix bibo: <http://purl.org/ontology/bibo/> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix foaf: <http://xmlns.com/foaf/0.1/> .
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sub:assertion {
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    schema:name "HEK293 cell mitochondrial transcriptome"@en ;
    sio:SIO_000228 300000000 ;
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  <http://purl.org/coloc-seq/experiment-human-mitochondria> schema:name "CoLoC-seq validation in human mitochondria"@en ;
    schema:result <http://purl.org/coloc-seq/result-mtdna-transcripts> , <http://purl.org/coloc-seq/result-ndna-contamination> ;
    schema:studySubject <http://purl.org/coloc-seq/dataset-hek293> ;
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    sio:SIO_000225 "100"^^xsd:float ;
    a schema:Thing ;
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    rdfs:comment "5.8S rRNA and U6 snRNA rapidly degraded in both CoLoC-seq and Mock CoLoC-seq"@en .
  <http://purl.org/coloc-seq/validation-correlation> schema:measurementTechnique "Spearman's correlation"@en ;
    schema:name "Validation against northern blotting"@en ;
    schema:statisticalSignificance "8.7e-73"^^xsd:float ;
    sio:SIO_000225 "0.888"^^xsd:float ;
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    rdfs:comment "90.6% of measurements within 0.3 of northern blotting values; high concordance of P0 values (ρ = 0.822, P = 1.6e-5)"@en .
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    schema:about <https://doi.org/10.1093/nar/gkac1205> ;
    schema:mentions <http://purl.org/coloc-seq/dataset-hek293> ;
    schema:text "CoLoC-seq validation in human mitochondria identified 351 transcripts, with mtDNA-encoded transcripts showing 100 percent protection and nDNA-encoded transcripts showing rapid degradation, demonstrating the method's accuracy."@en ;
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    schema:datePublished "2023"^^xsd:gYear ;
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    schema:name "CoLoC-seq probes the global topology of organelle transcriptomes"@en ;
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  sub:__1 schema:name "Nucleic Acids Research"@en ;
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   orcid:0009-0005-7752-8203 foaf:name "Tiado Kollender" .
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    rdfs:comment "AIDA-based assertion about CoLoC-seq validation and experimental results"@en ;
    rdfs:label "Nanopublication: CoLoC-seq validation results in human mitochondria"@en ;
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