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All rights reserved. http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/title BEL Framework Large Corpus Document http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/pav/authoredBy http://www.tkuhn.ch/bel2nanopub/RA6ZHbSTZ8FeNshit8nSh7fTZzFWkIRoEvXO57Qnn6_Ag#_4 http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/pav/version 20131211 http://www.tkuhn.ch/bel2nanopub/RA6ZHbSTZ8FeNshit8nSh7fTZzFWkIRoEvXO57Qnn6_Ag#_3 http://www.w3.org/ns/prov#value PURPOSE: To investigate genes that are up- and downregulated in rat retinal ischemia-reperfusion injury systematically by using an oligonucleotide microarray system and to determine temporal and spatial expression changes of some genes that showed upregulation in the analysis. METHODS: Retinal ischemia was induced in rats by increasing intraocular pressure to 110 mm Hg for 1 hour. Gene expression at 12 hours after reperfusion was compared with that in the control retina by using oligonucleotide microarrays that display a total of 8800 genes and expressed sequence tags (ESTs). Temporal and spatial expression changes of immediate early genes and cell-cycle-related genes were studied by using real-time polymerase chain reaction (PCR) and immunohistochemical methods. RESULTS: At 12 hours after reperfusion, 135 genes and ESTs were found to be up- or downregulated. The upregulated genes were classified into seven groups: (1) immediate early genes; (2) cell-cycle-related genes; (3) stress-responsive protein genes; (4) cell-signaling protein genes; (5) cell-adhesion and cell surface protein genes; (6) genes for translation and protein turnover; and (7) genes for metabolism. Real-time PCR analyses showed peaks of Fra-1 expression at 6 hours after reperfusion, whereas those for c-Jun, Jun B, and cyclin D1 were at 24 hours. Fra-1 and Jun B immunoreactivities were found in M??ller cells, whereas c-Jun and cyclin D1 immunoreactivities were found in apoptotic retinal neurons. CONCLUSIONS: Gene expression changes after a retinal ischemia-reperfusion injury were profiled by using an oligonucleotide microarray system. Seven groups of genes were found to be upregulated by the injury. Among the immediate early genes, Fra-1 and Jun B immunoreactivities were found in M??ller cells whereas c-Jun and cyclin D1 immunoreactivities were found in apoptotic retinal neurons. http://www.tkuhn.ch/bel2nanopub/RA6ZHbSTZ8FeNshit8nSh7fTZzFWkIRoEvXO57Qnn6_Ag#_3 http://www.w3.org/ns/prov#wasQuotedFrom http://www.ncbi.nlm.nih.gov/pubmed/12714663 http://www.tkuhn.ch/bel2nanopub/RA6ZHbSTZ8FeNshit8nSh7fTZzFWkIRoEvXO57Qnn6_Ag#_4 http://www.w3.org/2000/01/rdf-schema#label Selventa http://www.tkuhn.ch/bel2nanopub/RA6ZHbSTZ8FeNshit8nSh7fTZzFWkIRoEvXO57Qnn6_Ag#assertion http://www.w3.org/ns/prov#hadPrimarySource http://www.ncbi.nlm.nih.gov/pubmed/12714663 http://www.tkuhn.ch/bel2nanopub/RA6ZHbSTZ8FeNshit8nSh7fTZzFWkIRoEvXO57Qnn6_Ag#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://www.tkuhn.ch/bel2nanopub/RA6ZHbSTZ8FeNshit8nSh7fTZzFWkIRoEvXO57Qnn6_Ag#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://www.tkuhn.ch/bel2nanopub/RA6ZHbSTZ8FeNshit8nSh7fTZzFWkIRoEvXO57Qnn6_Ag#_3 http://www.tkuhn.ch/bel2nanopub/RA6ZHbSTZ8FeNshit8nSh7fTZzFWkIRoEvXO57Qnn6_Ag#pubinfo http://www.tkuhn.ch/bel2nanopub/RA6ZHbSTZ8FeNshit8nSh7fTZzFWkIRoEvXO57Qnn6_Ag http://purl.org/dc/terms/created 2014-07-03T14:32:01.427+02:00 http://www.tkuhn.ch/bel2nanopub/RA6ZHbSTZ8FeNshit8nSh7fTZzFWkIRoEvXO57Qnn6_Ag http://purl.org/pav/createdBy http://orcid.org/0000-0001-6818-334X http://www.tkuhn.ch/bel2nanopub/RA6ZHbSTZ8FeNshit8nSh7fTZzFWkIRoEvXO57Qnn6_Ag http://purl.org/pav/createdBy http://orcid.org/0000-0002-1267-0234