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All rights reserved. http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/title BEL Framework Large Corpus Document http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/pav/authoredBy http://www.tkuhn.ch/bel2nanopub/RA4tjWWSgDclN2CvtA7ZZybotgboP2pX6oKh-rJ5O1qWI#_5 http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/pav/version 20131211 http://www.tkuhn.ch/bel2nanopub/RA4tjWWSgDclN2CvtA7ZZybotgboP2pX6oKh-rJ5O1qWI#_4 http://www.w3.org/ns/prov#value We have studied the effect of enhanced expression of epithelial Na(+)channels (ENaC) on the ATP-induced Cl(-)secretion in the mouse epithelium using short-circuit current (I(SC)) and RT-PCR techniques. The amiloride sensitivity of basal current (I(b)) across the cultured endometrial epithelia was found to vary with the magnitude of the I(b), the higher the I(b)the greater its sensitivity to amiloride, indicating possible elevation of ENaC. However, the magnitude of ATP-induced I(SC), previously demonstrated to be mediated by Ca(2+)-activated chloride channel (CaCC), decreased as the amiloride sensitivity of the I(b)increased, suggesting a possible inhibitory effect of elevated expression of ENaC on ATP-mediated chloride secretion. The Matrigel treatment for culturing the endometrial epithelia affected the amiloride sensitivity of the I(b)as well as the ATP-induced I(SC)reversedly. Competitive RT-PCR demonstrated that the expression of both ENaC gamma subunits and CaCC was enhanced in Matrigel-treated cultures. However, the observed reduction in the ATP-induced or CaCC-mediated I(SC)could not be explained by the CaCC expression pattern. These data suggest that inhibition of CaCC function is due to enhanced ENaC expression. Therefore, in addition to interacting with CFTR, ENaC also appears to interact with CaCC in the mouse endometrial epithelium. Physiologically the present findings indicate that enhanced expression of ENaC leads to suppression of other Cl(-)channels, such as CFTR and CaCC, thereby preconditioning the endometrium in favour of overall salt and water absorption as observed during embryo implantation. http://www.tkuhn.ch/bel2nanopub/RA4tjWWSgDclN2CvtA7ZZybotgboP2pX6oKh-rJ5O1qWI#_4 http://www.w3.org/ns/prov#wasQuotedFrom http://www.ncbi.nlm.nih.gov/pubmed/11589620 http://www.tkuhn.ch/bel2nanopub/RA4tjWWSgDclN2CvtA7ZZybotgboP2pX6oKh-rJ5O1qWI#_5 http://www.w3.org/2000/01/rdf-schema#label Selventa http://www.tkuhn.ch/bel2nanopub/RA4tjWWSgDclN2CvtA7ZZybotgboP2pX6oKh-rJ5O1qWI#assertion http://www.w3.org/ns/prov#hadPrimarySource http://www.ncbi.nlm.nih.gov/pubmed/11589620 http://www.tkuhn.ch/bel2nanopub/RA4tjWWSgDclN2CvtA7ZZybotgboP2pX6oKh-rJ5O1qWI#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://www.tkuhn.ch/bel2nanopub/RA4tjWWSgDclN2CvtA7ZZybotgboP2pX6oKh-rJ5O1qWI#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://www.tkuhn.ch/bel2nanopub/RA4tjWWSgDclN2CvtA7ZZybotgboP2pX6oKh-rJ5O1qWI#_4 http://www.tkuhn.ch/bel2nanopub/RA4tjWWSgDclN2CvtA7ZZybotgboP2pX6oKh-rJ5O1qWI#pubinfo http://www.tkuhn.ch/bel2nanopub/RA4tjWWSgDclN2CvtA7ZZybotgboP2pX6oKh-rJ5O1qWI http://purl.org/dc/terms/created 2014-07-03T14:31:43.955+02:00 http://www.tkuhn.ch/bel2nanopub/RA4tjWWSgDclN2CvtA7ZZybotgboP2pX6oKh-rJ5O1qWI http://purl.org/pav/createdBy http://orcid.org/0000-0001-6818-334X http://www.tkuhn.ch/bel2nanopub/RA4tjWWSgDclN2CvtA7ZZybotgboP2pX6oKh-rJ5O1qWI http://purl.org/pav/createdBy http://orcid.org/0000-0002-1267-0234