. . . . "f(A) = (1 - P0) * exp(-k'i * A) + P0"@en . "CoLoC-seq Kinetics Model"@en . "P0"@en . "k'i"@en . . "P0 represents protected RNA pool proportion; k'i represents digestion rate constant"@en . . "Extracellular vesicle characterization"@en . "Mitochondrial transcriptomics"@en . "Organelle RNA localisation"@en . "Controlled Level of Contamination coupled to deep sequencing - a novel subcellular transcriptomics approach"@en . "CoLoC-seq"@en . . . . . "Leverages classical enzymatic kinetics to track depletion dynamics of transcripts using RNase gradients"@en . "Control experiment with detergent-permeabilized membranes to detect false positives"@en . "Mock CoLoC-seq"@en . . "Distinguishes genuine protection from intrinsic RNase resistance"@en . . "CoLoC-seq is a novel subcellular transcriptomics approach that uses enzymatic kinetics to distinguish genuine organellar resident RNAs from surface-attached contaminants."@en . . . "10.1093/nar/gkac1205" . "2023"^^ . . "CoLoC-seq probes the global topology of organelle transcriptomes"@en . . . "0305-1048" . "Nucleic Acids Research"@en . . . . . . "Tiado Kollender" . "2026-01-11T00:00:00Z"^^ . . . . . . . "AIDA-based assertion about CoLoC-seq as a novel subcellular transcriptomics approach"@en . "Nanopublication: CoLoC-seq method for subcellular RNA localisation"@en . . "RSA" . "MIIBIjANBgkqhkiG9w0BAQEFAAOCAQ8AMIIBCgKCAQEAoSR/rqhnkTPQO8u4l2JQAemJo5cN83rT54nA5NXHUqaeu26bEjT/rsX1PCVBhhJ3cV2uu4wjI0NfcVaKPKmODSf6p579tdOykOnjbWLbxxPMe5DC5eV1ThMHsFsNU/6vCYIfS00E7wre7fnFYz7FAG7v1MZEyWeSXsHgk9jHoqQQDPqup5PHReKp1dd6J0Nht/OfSYnUancKAgIFyPhkTlThsLK5xSNyNEgiTR7pwkP5pGq2AnD6bPw4G3kiPnIpIGZNsaeIOvhn64gwbrtoJVdoun/namw6FQ7StEC5km/0evaBZc4qIextwO/s32Wru77ukblJ0Xi/YzbC0iLuIQIDAQAB" . "DqoySgfA2Sjz1r73VRoMGw5kbFfrreqGsiYZQ1d7KQYSuVp+TiBZJX9ivuR+G8Z1WC/pQCqc62j7PQl+LTMO3OkQ/DMzPaxqZHM3I8NOWXZJuUEObXRAcQxHhpqO9Px9UWp8AWXyQ+jQEC5HWM+g9ggo6SVgZpDMKiJuQb1afnDZbcP0rHyOI6NwK5MaPAncpO8VgjE20lWprImn0uwp4wUVmJL6/+LINpPCm1T4WsFtj8aynLZs+nW3rfjxyY/Q2bUAjUfG8i5bnaW0Z3rUrGQOyP7OQiZG3egF2B9LluwNHzPQs3t3uwMeZSM6qNuO37rEshlxKLufFzjp7p1qTA==" . .