[ {
  "@graph" : [ {
    "@id" : "https://w3id.org/np/RA4TkcJzz1U1F2f3Mh29t1N0eYXyPFltTxbk9t0Y_v-AU",
    "@type" : [ "http://www.nanopub.org/nschema#Nanopublication" ],
    "http://www.nanopub.org/nschema#hasAssertion" : [ {
      "@id" : "https://w3id.org/np/RA4TkcJzz1U1F2f3Mh29t1N0eYXyPFltTxbk9t0Y_v-AU/assertion"
    } ],
    "http://www.nanopub.org/nschema#hasProvenance" : [ {
      "@id" : "https://w3id.org/np/RA4TkcJzz1U1F2f3Mh29t1N0eYXyPFltTxbk9t0Y_v-AU/provenance"
    } ],
    "http://www.nanopub.org/nschema#hasPublicationInfo" : [ {
      "@id" : "https://w3id.org/np/RA4TkcJzz1U1F2f3Mh29t1N0eYXyPFltTxbk9t0Y_v-AU/pubinfo"
    } ]
  } ],
  "@id" : "https://w3id.org/np/RA4TkcJzz1U1F2f3Mh29t1N0eYXyPFltTxbk9t0Y_v-AU/Head"
}, {
  "@graph" : [ {
    "@id" : "http://purl.org/coloc-seq/kinetics-model",
    "@type" : [ "http://schema.org/Thing" ],
    "http://schema.org/formula" : [ {
      "@language" : "en",
      "@value" : "f(A) = (1 - P0) * exp(-k'i * A) + P0"
    } ],
    "http://schema.org/name" : [ {
      "@language" : "en",
      "@value" : "CoLoC-seq Kinetics Model"
    } ],
    "http://semanticscience.org/resource/SIO_000211" : [ {
      "@language" : "en",
      "@value" : "P0"
    }, {
      "@language" : "en",
      "@value" : "k'i"
    } ],
    "http://www.w3.org/2000/01/rdf-schema#comment" : [ {
      "@language" : "en",
      "@value" : "P0 represents protected RNA pool proportion; k'i represents digestion rate constant"
    } ]
  }, {
    "@id" : "http://purl.org/coloc-seq/method",
    "@type" : [ "http://edamontology.org/data_0957", "http://schema.org/Thing" ],
    "http://edamontology.org/has_function" : [ {
      "@id" : "http://edamontology.org/operation_3680"
    } ],
    "http://schema.org/applicationArea" : [ {
      "@language" : "en",
      "@value" : "Extracellular vesicle characterization"
    }, {
      "@language" : "en",
      "@value" : "Mitochondrial transcriptomics"
    }, {
      "@language" : "en",
      "@value" : "Organelle RNA localisation"
    } ],
    "http://schema.org/description" : [ {
      "@language" : "en",
      "@value" : "Controlled Level of Contamination coupled to deep sequencing - a novel subcellular transcriptomics approach"
    } ],
    "http://schema.org/name" : [ {
      "@language" : "en",
      "@value" : "CoLoC-seq"
    } ],
    "http://semanticscience.org/resource/SIO_000135" : [ {
      "@id" : "http://purl.org/coloc-seq/kinetics-model"
    }, {
      "@id" : "http://purl.org/coloc-seq/mock-coloc-experiment"
    } ],
    "http://www.w3.org/2000/01/rdf-schema#comment" : [ {
      "@language" : "en",
      "@value" : "Leverages classical enzymatic kinetics to track depletion dynamics of transcripts using RNase gradients"
    } ]
  }, {
    "@id" : "http://purl.org/coloc-seq/mock-coloc-experiment",
    "@type" : [ "http://schema.org/Thing" ],
    "http://schema.org/description" : [ {
      "@language" : "en",
      "@value" : "Control experiment with detergent-permeabilized membranes to detect false positives"
    } ],
    "http://schema.org/name" : [ {
      "@language" : "en",
      "@value" : "Mock CoLoC-seq"
    } ],
    "http://www.w3.org/2000/01/rdf-schema#comment" : [ {
      "@language" : "en",
      "@value" : "Distinguishes genuine protection from intrinsic RNase resistance"
    } ]
  }, {
    "@id" : "https://w3id.org/np/RA4TkcJzz1U1F2f3Mh29t1N0eYXyPFltTxbk9t0Y_v-AU",
    "@type" : [ "https://w3id.org/np/RA4TkcJzz1U1F2f3Mh29t1N0eYXyPFltTxbk9t0Y_v-AU/o/ntemplate/AIDA-Sentence" ],
    "http://schema.org/about" : [ {
      "@id" : "https://doi.org/10.1093/nar/gkac1205"
    } ],
    "http://schema.org/text" : [ {
      "@language" : "en",
      "@value" : "CoLoC-seq is a novel subcellular transcriptomics approach that uses enzymatic kinetics to distinguish genuine organellar resident RNAs from surface-attached contaminants."
    } ],
    "http://www.w3.org/2000/01/rdf-schema#seeAlso" : [ {
      "@id" : "http://edamontology.org/operation_3680"
    } ]
  } ],
  "@id" : "https://w3id.org/np/RA4TkcJzz1U1F2f3Mh29t1N0eYXyPFltTxbk9t0Y_v-AU/assertion"
}, {
  "@graph" : [ {
    "@id" : "https://doi.org/10.1093/nar/gkac1205",
    "@type" : [ "http://purl.org/ontology/bibo/AcademicArticle", "http://schema.org/ScholarlyArticle" ],
    "http://purl.org/ontology/bibo/doi" : [ {
      "@value" : "10.1093/nar/gkac1205"
    } ],
    "http://schema.org/datePublished" : [ {
      "@type" : "http://www.w3.org/2001/XMLSchema#gYear",
      "@value" : "2023"
    } ],
    "http://schema.org/isPartOf" : [ {
      "@id" : "https://w3id.org/np/RA4TkcJzz1U1F2f3Mh29t1N0eYXyPFltTxbk9t0Y_v-AU/__1"
    } ],
    "http://schema.org/name" : [ {
      "@language" : "en",
      "@value" : "CoLoC-seq probes the global topology of organelle transcriptomes"
    } ]
  }, {
    "@id" : "https://w3id.org/np/RA4TkcJzz1U1F2f3Mh29t1N0eYXyPFltTxbk9t0Y_v-AU/__1",
    "@type" : [ "http://schema.org/Periodical" ],
    "http://schema.org/issn" : [ {
      "@value" : "0305-1048"
    } ],
    "http://schema.org/name" : [ {
      "@language" : "en",
      "@value" : "Nucleic Acids Research"
    } ]
  }, {
    "@id" : "https://w3id.org/np/RA4TkcJzz1U1F2f3Mh29t1N0eYXyPFltTxbk9t0Y_v-AU/assertion",
    "http://www.w3.org/ns/prov#wasAttributedTo" : [ {
      "@id" : "https://orcid.org/0000-0001-8763-6703"
    }, {
      "@id" : "https://orcid.org/0000-0002-1481-2996"
    }, {
      "@id" : "https://orcid.org/0000-0002-9142-7979"
    } ],
    "http://www.w3.org/ns/prov#wasDerivedFrom" : [ {
      "@id" : "https://doi.org/10.1093/nar/gkac1205"
    } ]
  } ],
  "@id" : "https://w3id.org/np/RA4TkcJzz1U1F2f3Mh29t1N0eYXyPFltTxbk9t0Y_v-AU/provenance"
}, {
  "@graph" : [ {
    "@id" : "https://orcid.org/0009-0005-7752-8203",
    "http://xmlns.com/foaf/0.1/name" : [ {
      "@value" : "Tiado Kollender"
    } ]
  }, {
    "@id" : "https://w3id.org/np/RA4TkcJzz1U1F2f3Mh29t1N0eYXyPFltTxbk9t0Y_v-AU",
    "@type" : [ "http://purl.org/nanopub/x/ExampleNanopub" ],
    "http://purl.org/dc/terms/created" : [ {
      "@type" : "http://www.w3.org/2001/XMLSchema#dateTime",
      "@value" : "2026-01-11T00:00:00Z"
    } ],
    "http://purl.org/dc/terms/license" : [ {
      "@id" : "https://creativecommons.org/publicdomain/zero/1.0/"
    } ],
    "http://purl.org/nanopub/x/signedBy" : [ {
      "@id" : "https://orcid.org/0009-0005-7752-8203"
    } ],
    "http://purl.org/nanopub/x/wasCreatedFromPubinfoTemplate" : [ {
      "@id" : "https://w3id.org/np/RA0J4vUn_dekg-U1kK3AOEt02p9mT2WO03uGxLDec1jLw"
    }, {
      "@id" : "https://w3id.org/np/RAukAcWHRDlkqxk7H2XNSegc1WnHI569INvNr-xdptDGI"
    } ],
    "http://purl.org/pav/createdBy" : [ {
      "@id" : "https://orcid.org/0009-0005-7752-8203"
    } ],
    "http://www.w3.org/2000/01/rdf-schema#comment" : [ {
      "@language" : "en",
      "@value" : "AIDA-based assertion about CoLoC-seq as a novel subcellular transcriptomics approach"
    } ],
    "http://www.w3.org/2000/01/rdf-schema#label" : [ {
      "@language" : "en",
      "@value" : "Nanopublication: CoLoC-seq method for subcellular RNA localisation"
    } ],
    "http://www.w3.org/ns/prov#wasAttributedTo" : [ {
      "@id" : "https://orcid.org/0009-0005-7752-8203"
    } ]
  }, {
    "@id" : "https://w3id.org/np/RA4TkcJzz1U1F2f3Mh29t1N0eYXyPFltTxbk9t0Y_v-AU/sig",
    "http://purl.org/nanopub/x/hasAlgorithm" : [ {
      "@value" : "RSA"
    } ],
    "http://purl.org/nanopub/x/hasPublicKey" : [ {
      "@value" : "MIIBIjANBgkqhkiG9w0BAQEFAAOCAQ8AMIIBCgKCAQEAoSR/rqhnkTPQO8u4l2JQAemJo5cN83rT54nA5NXHUqaeu26bEjT/rsX1PCVBhhJ3cV2uu4wjI0NfcVaKPKmODSf6p579tdOykOnjbWLbxxPMe5DC5eV1ThMHsFsNU/6vCYIfS00E7wre7fnFYz7FAG7v1MZEyWeSXsHgk9jHoqQQDPqup5PHReKp1dd6J0Nht/OfSYnUancKAgIFyPhkTlThsLK5xSNyNEgiTR7pwkP5pGq2AnD6bPw4G3kiPnIpIGZNsaeIOvhn64gwbrtoJVdoun/namw6FQ7StEC5km/0evaBZc4qIextwO/s32Wru77ukblJ0Xi/YzbC0iLuIQIDAQAB"
    } ],
    "http://purl.org/nanopub/x/hasSignature" : [ {
      "@value" : "DqoySgfA2Sjz1r73VRoMGw5kbFfrreqGsiYZQ1d7KQYSuVp+TiBZJX9ivuR+G8Z1WC/pQCqc62j7PQl+LTMO3OkQ/DMzPaxqZHM3I8NOWXZJuUEObXRAcQxHhpqO9Px9UWp8AWXyQ+jQEC5HWM+g9ggo6SVgZpDMKiJuQb1afnDZbcP0rHyOI6NwK5MaPAncpO8VgjE20lWprImn0uwp4wUVmJL6/+LINpPCm1T4WsFtj8aynLZs+nW3rfjxyY/Q2bUAjUfG8i5bnaW0Z3rUrGQOyP7OQiZG3egF2B9LluwNHzPQs3t3uwMeZSM6qNuO37rEshlxKLufFzjp7p1qTA=="
    } ],
    "http://purl.org/nanopub/x/hasSignatureTarget" : [ {
      "@id" : "https://w3id.org/np/RA4TkcJzz1U1F2f3Mh29t1N0eYXyPFltTxbk9t0Y_v-AU"
    } ]
  } ],
  "@id" : "https://w3id.org/np/RA4TkcJzz1U1F2f3Mh29t1N0eYXyPFltTxbk9t0Y_v-AU/pubinfo"
} ]