@prefix obo: <http://purl.obolibrary.org/obo/> .
@prefix dce: <http://purl.org/dc/elements/1.1/> .
@prefix pplan: <http://purl.org/net/p-plan#> .
@prefix this: <http://purl.org/np/RA3YD3Pt8TUVEX9idVVTqPkqjN8Pqe0j2KkUhBFOYt0uM> .
@prefix sub: <http://purl.org/np/RA3YD3Pt8TUVEX9idVVTqPkqjN8Pqe0j2KkUhBFOYt0uM#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix sp: <http://purl.org/net/SMARTprotocol#> .
sub:Head {
   this: np:hasAssertion sub:assertion ;
    np:hasProvenance sub:provenance ;
    np:hasPublicationInfo sub:pubInfo ;
    a np:Nanopublication .
}
sub:assertion {
   obo:PO_0025034 rdfs:label "leaf tissues" .
  sub:_1 rdfs:label "extraction buffer" .
  sub:step dce:description "Take one gram of frozen finely powdered leaf tissues in a new 50 mL Falcon tube and mixed with the pre-heated extraction buffer (10 ml) for one sample. Breifly, vertex for 30sec to ensure leaf material is fully mixed with buffer. Fine grind is a key to obtaining high DNA quantity with lesser artifacts of resin." ;
    sp:hasExperimentalInput obo:PO_0025034 ;
    a pplan:Step ;
    bs:bioSampleUsed obo:PO_0025034 ;
    bs:labEquipmentUsed <http://www.bioassayontology.org/bao#BAO_0010014> ;
    bs:reagentUsed sub:_1 .
  <http://www.bioassayontology.org/bao#BAO_0010014> rdfs:label "50 mL Falcon tube" .
}
sub:provenance {
   sub:assertion pav:createdWith <https://nanotate.bitsfetch.com> ;
    prov:generatedAtTime "2021-03-11T16:45:43.287006"^^xsd:dateTime ;
    prov:wasAttributedTo <https://hypothes.is/users/iannotate> .
}
sub:pubInfo {
   sub:assertion prov:wasDerivedFrom authority:6TwtXIKuEeufVs8lGVSnJg , authority:_rye-oKuEeuS7bc4SYlicg , authority:quasvIKuEeuXbCtzLANu_g , authority:zpbWHoKuEeujimtnDxFfPQ .
  sub:sig npx:hasAlgorithm "RSA" ;
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB" ;
    npx:hasSignature "AlkRqY3aHl5OD/+YmByHNB4OgJ5aSDjD360S8i0agflM75D5aBH8zIjAZn0ijQTrKYoX1cNWeqaoVpOZ3zbNZCa3jxVRzcdMx1HjR0dnaBSNERs+vLHYTpMEUeIdqzyOv1MXBESeVkVinH+CrJLEwxAkBitb7Sz3d4UiHLS2BkU=" ;
    npx:hasSignatureTarget this: .
  this: npx:introduces sub:step ;
    prov:generatedAtTime "2021-03-11T16:45:43.287006"^^xsd:dateTime ;
    prov:wasAttributedTo <https://hypothes.is/users/iannotate> .
}