http://www.tkuhn.ch/bel2nanopub/RA33CjvdEwlnXmu434wSqCX3r_X7qharArzqeZWuSteME#Head http://www.tkuhn.ch/bel2nanopub/RA33CjvdEwlnXmu434wSqCX3r_X7qharArzqeZWuSteME http://www.nanopub.org/nschema#hasAssertion http://www.tkuhn.ch/bel2nanopub/RA33CjvdEwlnXmu434wSqCX3r_X7qharArzqeZWuSteME#assertion http://www.tkuhn.ch/bel2nanopub/RA33CjvdEwlnXmu434wSqCX3r_X7qharArzqeZWuSteME http://www.nanopub.org/nschema#hasProvenance http://www.tkuhn.ch/bel2nanopub/RA33CjvdEwlnXmu434wSqCX3r_X7qharArzqeZWuSteME#provenance http://www.tkuhn.ch/bel2nanopub/RA33CjvdEwlnXmu434wSqCX3r_X7qharArzqeZWuSteME http://www.nanopub.org/nschema#hasPublicationInfo http://www.tkuhn.ch/bel2nanopub/RA33CjvdEwlnXmu434wSqCX3r_X7qharArzqeZWuSteME#pubinfo http://www.tkuhn.ch/bel2nanopub/RA33CjvdEwlnXmu434wSqCX3r_X7qharArzqeZWuSteME http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.nanopub.org/nschema#Nanopublication http://www.tkuhn.ch/bel2nanopub/RA33CjvdEwlnXmu434wSqCX3r_X7qharArzqeZWuSteME#assertion http://www.tkuhn.ch/bel2nanopub/RA33CjvdEwlnXmu434wSqCX3r_X7qharArzqeZWuSteME#_1 http://purl.obolibrary.org/obo/BFO_0000051 http://www.genenames.org/cgi-bin/gene_symbol_report?hgnc_id=3467 http://www.tkuhn.ch/bel2nanopub/RA33CjvdEwlnXmu434wSqCX3r_X7qharArzqeZWuSteME#_1 http://purl.obolibrary.org/obo/BFO_0000051 http://www.tkuhn.ch/bel2nanopub/RA33CjvdEwlnXmu434wSqCX3r_X7qharArzqeZWuSteME#_2 http://www.tkuhn.ch/bel2nanopub/RA33CjvdEwlnXmu434wSqCX3r_X7qharArzqeZWuSteME#_1 http://purl.obolibrary.org/obo/BFO_0000066 http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=9606 http://www.tkuhn.ch/bel2nanopub/RA33CjvdEwlnXmu434wSqCX3r_X7qharArzqeZWuSteME#_1 http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://amigo.geneontology.org/amigo/term/GO:0043234 http://www.tkuhn.ch/bel2nanopub/RA33CjvdEwlnXmu434wSqCX3r_X7qharArzqeZWuSteME#_2 http://purl.obolibrary.org/obo/RO_0002204 http://www.genenames.org/cgi-bin/gene_symbol_report?hgnc_id=4983 http://www.tkuhn.ch/bel2nanopub/RA33CjvdEwlnXmu434wSqCX3r_X7qharArzqeZWuSteME#_2 http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.ebi.ac.uk/chebi/searchId.do?chebiId=CHEBI_36080 http://www.tkuhn.ch/bel2nanopub/RA33CjvdEwlnXmu434wSqCX3r_X7qharArzqeZWuSteME#assertion http://www.w3.org/2000/01/rdf-schema#label complex(p(HGNC:HMGB1),g(HGNC:ESR1)) http://www.tkuhn.ch/bel2nanopub/RA33CjvdEwlnXmu434wSqCX3r_X7qharArzqeZWuSteME#provenance http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/description Approximately 61,000 statements. http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/rights Copyright (c) 2011-2012, Selventa. All rights reserved. http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/title BEL Framework Large Corpus Document http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/pav/authoredBy http://www.tkuhn.ch/bel2nanopub/RA33CjvdEwlnXmu434wSqCX3r_X7qharArzqeZWuSteME#_4 http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/pav/version 1.4 http://www.tkuhn.ch/bel2nanopub/RA33CjvdEwlnXmu434wSqCX3r_X7qharArzqeZWuSteME#_3 http://www.w3.org/ns/prov#value The effect of high-mobility group (HMG) protein HMG-1 and purified recombinant TATA-binding protein-associated factor TAF(II)30 on ER-ERE binding and transcription initiation were assessed by electrophoretic mobility shift assay and in vitro transcription from an ERE-containing template (pERE2LovTATA), respectively. We find that purified, recombinant ER fails to bind to ERE in spite of high ligand-binding activity and electrophoretic and immunological properties identical to ER in MCF-7 breast cancer cells. HMG-1 interacts with ER and promotes ER-ERE binding in a concentration- and time-dependent manner.We find that TAF(II)30 has no effect on ER-ERE binding either alone or in combination with ER and HMG-1. Although HMG-1 promotes ER-ERE binding, it fails to stimulate transcription initiation either in the presence or absence of hormone. In contrast, TAF(II)30, while not affecting ER-ERE binding, stimulates transcription initiation 20-fold in the presence of HMG-1. http://www.tkuhn.ch/bel2nanopub/RA33CjvdEwlnXmu434wSqCX3r_X7qharArzqeZWuSteME#_3 http://www.w3.org/ns/prov#wasQuotedFrom http://www.ncbi.nlm.nih.gov/pubmed/9212049 http://www.tkuhn.ch/bel2nanopub/RA33CjvdEwlnXmu434wSqCX3r_X7qharArzqeZWuSteME#_4 http://www.w3.org/2000/01/rdf-schema#label Selventa http://www.tkuhn.ch/bel2nanopub/RA33CjvdEwlnXmu434wSqCX3r_X7qharArzqeZWuSteME#assertion http://www.w3.org/ns/prov#hadPrimarySource http://www.ncbi.nlm.nih.gov/pubmed/9212049 http://www.tkuhn.ch/bel2nanopub/RA33CjvdEwlnXmu434wSqCX3r_X7qharArzqeZWuSteME#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://www.tkuhn.ch/bel2nanopub/RA33CjvdEwlnXmu434wSqCX3r_X7qharArzqeZWuSteME#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://www.tkuhn.ch/bel2nanopub/RA33CjvdEwlnXmu434wSqCX3r_X7qharArzqeZWuSteME#_3 http://www.tkuhn.ch/bel2nanopub/RA33CjvdEwlnXmu434wSqCX3r_X7qharArzqeZWuSteME#pubinfo http://www.tkuhn.ch/bel2nanopub/RA33CjvdEwlnXmu434wSqCX3r_X7qharArzqeZWuSteME http://purl.org/dc/terms/created 2014-07-03T14:30:58.580+02:00 http://www.tkuhn.ch/bel2nanopub/RA33CjvdEwlnXmu434wSqCX3r_X7qharArzqeZWuSteME http://purl.org/pav/createdBy http://orcid.org/0000-0001-6818-334X http://www.tkuhn.ch/bel2nanopub/RA33CjvdEwlnXmu434wSqCX3r_X7qharArzqeZWuSteME http://purl.org/pav/createdBy http://orcid.org/0000-0002-1267-0234