sub:provenance {
  beldoc: dce:description "Approximately 61,000 statements." ;
    dce:rights "Copyright (c) 2011-2012, Selventa. All rights reserved." ;
    dce:title "BEL Framework Large Corpus Document" ;
    pav:authoredBy sub:_6 ;
    pav:version "1.4" .
  sub:_5 prov:value "we transiently transfected the wild-type and CD40 cytoplasmic domain mutants with a plasmid containing the NF-kappaB response element upstream of a luciferase gene (pNF-kappaB-luc) and 2 ug of a Renilla luciferase (pHRL-luc) plasmid to normalize for transfection efficiency. The cells were subsequently stimulated via CD40 using CHO-CD154 for 16 h. Stimulation of both the wild-type and hCD 32 mutant resulted in a 3-fold induction of NF-kappaB luciferase activity above background, whereas stimulation of the hCD40EEAA mutant did not result in detectable stimulation of NF-kappaB reporter activity (Fig. 9A). Thus, optimal NF-kappaB activation requires an intact TRAF6 binding domain of CD40." ;
    prov:wasQuotedFrom pubmed:15634933 .
  sub:_6 rdfs:label "Selventa" .
  sub:assertion prov:hadPrimarySource pubmed:15634933 ;
    prov:wasDerivedFrom beldoc: , sub:_5 .
}