@prefix dce: <http://purl.org/dc/elements/1.1/> .
@prefix pplan: <http://purl.org/net/p-plan#> .
@prefix this: <http://purl.org/np/RA1nHbxUGfjEyKLYgzLha1EyQ4dCQgi-bDw3t1zymO8nQ> .
@prefix sub: <http://purl.org/np/RA1nHbxUGfjEyKLYgzLha1EyQ4dCQgi-bDw3t1zymO8nQ#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix sp: <http://purl.org/net/SMARTprotocol#> .

sub:Head {
  this: np:hasAssertion sub:assertion;
    np:hasProvenance sub:provenance;
    np:hasPublicationInfo sub:pubInfo;
    a np:Nanopublication .
}

sub:assertion {
  sub:_1 rdfs:label "PBMCs" .
  
  sub:_2 rdfs:label "PBMCs" .
  
  sub:_3 rdfs:label "cells " .
  
  sub:_4 rdfs:label "tubes" .
  
  sub:step dce:description "The cells in interphase need to be aspirated without delay. If the tubes are kept standing for more than 10 min, PBMCs from the interphase will get disturbed and start settling down.";
    sp:hasExperimentalOutput sub:_1;
    a pplan:Step;
    bs:bioSampleUsed sub:_2, sub:_3;
    bs:labEquipmentUsed sub:_4 .
}

sub:provenance {
  sub:assertion pav:createdWith <https://nanotate.bitsfetch.com>;
    prov:generatedAtTime "2021-02-14T04:55:00.251577"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/zuluaga.lizzz> .
}

sub:pubInfo {
  sub:assertion prov:wasDerivedFrom authority:VmZAllbsEeu23IPW9adrlw, authority:eJV2ClbsEeukJVdEMvbxZg,
      authority:lNhRwFbsEeuO5c-U_xj-fA, authority:r8UpBFbsEeudox_3n1y1Bg .
  
  sub:sig npx:hasAlgorithm "RSA";
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB";
    npx:hasSignature "arUz0s1E71P8PTzKHaJPCIiRJRcIXHNCOZKlMZ1L972IXMXQuzpsjBnuB907CavbjRGgwmP699Ppqugt8pK9SL2VfUYch7NNkC3lmrpJq/unaoFXvkowgQltqZXtC/w5gPyQDVyPpQ+VzNaxfgETtMNezmibQWQPCZbnYMeCAkU=";
    npx:hasSignatureTarget this: .
  
  this: npx:introduces sub:step;
    prov:generatedAtTime "2021-02-14T04:55:00.251577"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/zuluaga.lizzz> .
}